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PMID: 10628975 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of mitotic homeologous recombination in yeast. Functions of mismatch repair and nucleotide excision repair genes.

Genetics ·Vol. 154 ·No. 1 ·2000-01-00 ·Pages 133-46

Nicholson A, Hendrix M, Jinks-Robertson S, Crouse GF

Abstract

The Saccharomyces cerevisiae homologs of the bacterial mismatch repair proteins MutS and MutL correct replication errors and prevent recombination between homeologous (nonidentical) sequences. Previously, we demonstrated that Msh2p, Msh3p, and Pms1p regulate recombination between 91% identical inverted repeats, and here use the same substrates to show that Mlh1p and Msh6p have important antirecombination roles. In addition, substrates containing defined types of mismatches (base-base mismatches; 1-, 4-, or 12-nt insertion/deletion loops; or 18-nt palindromes) were used to examine recognition of these mismatches in mitotic recombination intermediates. Msh2p was required for recognition of all types of mismatches, whereas Msh6p recognized only base-base mismatches and 1-nt insertion/deletion loops. Msh3p was involved in recognition of the palindrome and all loops, but also had an unexpected antirecombination role when the potential heteroduplex contained only base-base mismatches. In contrast to their similar antimutator roles, Pms1p consistently inhibited recombination to a lesser degree than did Msh2p. In addition to the yeast MutS and MutL homologs, the exonuclease Exo1p and the nucleotide excision repair proteins Rad1p and Rad10p were found to have roles in inhibiting recombination between mismatched substrates.

MeSH Terms
Base Pair Mismatch/genetics Base Sequence DNA Primers DNA Repair/genetics Epistasis, Genetic Mitosis/genetics Recombination, Genetic Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/cytology,genetics
Chemicals
DNA Primers
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nicholson A
Graduate Program in Genetics and Molecular Biology, Emory University, Atlanta, Georgia 30322, USA.
Hendrix M
Jinks-Robertson S
Crouse G F
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/154.1.133
Published
2000-01-00
Pages
133-46
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460927
Subset
IM
Grants
NCI NIH HHS · CA54050 · United States
NIGMS NIH HHS · GM38464 · United States
External Links
PubMed source DOI: 10.1093/genetics/154.1.133 PMC Full Text View journal details
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