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PMID: 1059128 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fatty acids as modulators of membrane functions: catecholamine-activated adenylate cyclase of the turkey erythrocyte.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 9 ·1975-09-00 ·Pages 3433-7

Orly J, Schramm M

Abstract

Activation of the adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1[ from turkey erythrocytes by isoproterenol decreased precipitously below 26 degrees. Certain unsaturated fatty acids enhanced the activation by isoproterenol up to 25-fold at reduced temperatures. The fatty acid also enhanced the formation of a persistent active state of the enzyme which was produced by preincubation with guanosine 5'-(beta,gamma-imino)triphosphate [Gpp(NH)p]. Once the enzyme had been activated by Gpp(NH)p plus isoproterenol the reaction rate was no longer as temperature sensitive and the fatty acid had little effect. The synthetic Gpp(NH)p apparently substituted for the natural GTP, which is known to play a regulatory role in the adenylate cyclase system. The findings suggest that the function of GTP which is mediated by the hormone is the temperature-sensitive event which is enhanced by the fatty acid. The use of free fatty acid to probe membrane-associated reactions in intact cells and in isolated membrane preparations is proposed.

MeSH Terms
Adenylyl Cyclases/metabolism Animals Cell Membrane/drug effects,enzymology Drug Synergism Enzyme Activation/drug effects Erythrocytes/drug effects,enzymology,ultrastructure Fatty Acids/pharmacology Guanosine Triphosphate/analogs & derivatives,pharmacology Isoproterenol/pharmacology Structure-Activity Relationship Temperature Time Factors
Chemicals
Fatty Acids Guanosine Triphosphate Adenylyl Cyclases Isoproterenol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Orly J
Schramm M
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.9.3433
Published
1975-09-00
Pages
3433-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433008
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.9.3433 PMC Full Text View journal details
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