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PMID: 10590153 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of a bovine coronavirus packaging signal.

Journal of virology ·Vol. 74 ·No. 1 ·2000-01-00 ·Pages 580-3

Cologna R, Hogue BG

Abstract

A region of the bovine coronavirus (BCV) genome that functions as a packaging signal has been cloned. The 291-nucleotide clone shares 72% homology with the region of mouse hepatitis coronavirus (MHV) gene 1b that contains the packaging signal. RNA transcripts were packaged into both BCV and MHV virions when the cloned region was appended to a noncoronavirus RNA. This is the first identification of a BCV packaging signal. The data demonstrate that the BCV genome contains a sequence that is conserved at both the sequence and functional levels, thus broadening our insight into coronavirus packaging.

MeSH Terms
Animals Base Sequence Blotting, Northern Cattle Chloramphenicol O-Acetyltransferase/genetics Cloning, Molecular Coronavirus, Bovine/genetics Genome, Viral Mice Molecular Sequence Data Nucleic Acid Conformation RNA, Viral/chemistry,genetics Sequence Homology, Nucleic Acid Virus Assembly/genetics
Chemicals
RNA, Viral Chloramphenicol O-Acetyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cologna R
Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030, USA.
Hogue B G
References (28)
28 references, click to expand
  1. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8122-6 PMID: 3095828
  2. Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure.
    J Mol Biol. 1999 May 21;288(5):911-40 PMID: 10329189
  3. Interactions between coronavirus nucleocapsid protein and viral RNAs: implications for viral transcription.
    J Virol. 1988 Nov;62(11):4280-7 PMID: 2845140
  4. Specific interaction between coronavirus leader RNA and nucleocapsid protein.
    J Virol. 1988 Nov;62(11):4288-95 PMID: 2845141
  5. Coronavirus subgenomic minus-strand RNAs and the potential for mRNA replicons.
    Proc Natl Acad Sci U S A. 1989 Jul;86(14):5626-30 PMID: 2546161
  6. Structure and orientation of expressed bovine coronavirus hemagglutinin-esterase protein.
    J Virol. 1990 Apr;64(4):1834-8 PMID: 2319653
  7. Deduced sequence of the bovine coronavirus spike protein and identification of the internal proteolytic cleavage site.
    Virology. 1990 May;176(1):296-301 PMID: 2184576
  8. The primary structure and expression of the second open reading frame of the polymerase gene of the coronavirus MHV-A59; a highly conserved polymerase is expressed by an efficient ribosomal frameshifting mechanism.
    Nucleic Acids Res. 1990 Apr 11;18(7):1825-32 PMID: 2159623
  9. Sequence and expression analysis of potential nonstructural proteins of 4.9, 4.8, 12.7, and 9.5 kDa encoded between the spike and membrane protein genes of the bovine coronavirus.
    Virology. 1990 Aug;177(2):488-95 PMID: 2142556
  10. Bovine coronavirus mRNA replication continues throughout persistent infection in cell culture.
    J Virol. 1990 Sep;64(9):4108-14 PMID: 2384915
  11. Analysis of efficiently packaged defective interfering RNAs of murine coronavirus: localization of a possible RNA-packaging signal.
    J Virol. 1990 Dec;64(12):6045-53 PMID: 2243386
  12. Alteration of the pH dependence of coronavirus-induced cell fusion: effect of mutations in the spike glycoprotein.
    J Virol. 1991 Apr;65(4):1916-28 PMID: 1848311
  13. A domain at the 3' end of the polymerase gene is essential for encapsidation of coronavirus defective interfering RNAs.
    J Virol. 1991 Jun;65(6):3219-26 PMID: 2033672
  14. Identification and characterization of a coronavirus packaging signal.
    J Virol. 1992 Jun;66(6):3522-30 PMID: 1316465
  15. Infectious defective interfering particles of VSV from transcripts of a cDNA clone.
    Cell. 1992 Jun 12;69(6):1011-20 PMID: 1318785
  16. The fitness of defective interfering murine coronavirus DI-a and its derivatives is decreased by nonsense and frameshift mutations.
    J Virol. 1992 Oct;66(10):5898-905 PMID: 1326650
  17. Presence of subgenomic mRNAs in virions of coronavirus IBV.
    Virology. 1993 Sep;196(1):172-8 PMID: 8395112
  18. Characterization of a replicating and packaged defective RNA of avian coronavirus infectious bronchitis virus.
    Virology. 1994 Sep;203(2):286-93 PMID: 8053152
  19. A cis-acting function for the coronavirus leader in defective interfering RNA replication.
    J Virol. 1994 Dec;68(12):8223-31 PMID: 7966615
  20. Molecular characterization of transmissible gastroenteritis coronavirus defective interfering genomes: packaging and heterogeneity.
    Virology. 1996 Mar 15;217(2):495-507 PMID: 8610441
  21. Identification of a region in the Sindbis virus nucleocapsid protein that is involved in specificity of RNA encapsidation.
    J Virol. 1996 May;70(5):2757-63 PMID: 8627749
  22. Replication and packaging of coronavirus infectious bronchitis virus defective RNAs lacking a long open reading frame.
    J Virol. 1996 Dec;70(12):8660-8 PMID: 8970992
  23. Murine coronavirus packaging signal confers packaging to nonviral RNA.
    J Virol. 1997 Jan;71(1):824-7 PMID: 8985424
  24. A subgenomic mRNA transcript of the coronavirus mouse hepatitis virus strain A59 defective interfering (DI) RNA is packaged when it contains the DI packaging signal.
    J Virol. 1997 Jul;71(7):5684-7 PMID: 9188649
  25. The molecular biology of coronaviruses.
    Adv Virus Res. 1997;48:1-100 PMID: 9233431
  26. Protein interactions during coronavirus assembly.
    J Virol. 1997 Dec;71(12):9278-84 PMID: 9371586
  27. Replication and packaging of transmissible gastroenteritis coronavirus-derived synthetic minigenomes.
    J Virol. 1999 Feb;73(2):1535-45 PMID: 9882359
  28. Sequence analysis of the bovine coronavirus nucleocapsid and matrix protein genes.
    Virology. 1987 Mar;157(1):47-57 PMID: 3029965
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.74.1.580-583.2000
Published
2000-01-00
Pages
580-3
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC111575
Subset
IM
Grants
NIAID NIH HHS · T32 AI007471 · United States
NIAID NIH HHS · AI07471 · United States
NIAID NIH HHS · AI33500 · United States
Databases
GENBANK
AF159126
External Links
PubMed source DOI: 10.1128/jvi.74.1.580-583.2000 PMC Full Text View journal details
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