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PMID: 8985424 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Murine coronavirus packaging signal confers packaging to nonviral RNA.

Journal of virology ·Vol. 71 ·No. 1 ·1997-01-00 ·Pages 824-7

Woo K, Joo M, Narayanan K, Kim KH, Makino S

Abstract

Studies of defective interfering (DI) RNAs of the murine coronavirus mouse hepatitis virus (MHV) suggest that a 69-nucleotide-long packaging signal is necessary for MHV genomic RNA packaging into MHV particles. In this study we showed that when RNA transcripts that consisted of a non-MHV sequence and the packaging signal were expressed in MHV-infected cells, they were packaged into MHV particles. Those RNA transcripts that lacked the packaging signal or those containing a mutated packaging signal did not package efficiently. Thus, the presence of the packaging signal was sufficient for RNA packaging into MHV particles.

MeSH Terms
Animals Cell Line Murine hepatitis virus/genetics,physiology RNA/metabolism Ribonuclease, Pancreatic/metabolism Signal Transduction Virion/metabolism Virus Assembly/physiology
Chemicals
RNA Ribonuclease, Pancreatic
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Woo K
Department of Microbiology, The University of Texas at Austin, 78712-1095, USA.
Joo M
Narayanan K
Kim K H
Makino S
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.71.1.824-827.1997
Published
1997-01-00
Pages
824-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC191125
Subset
IM
Grants
NIAID NIH HHS · AI29984 · United States
NIAID NIH HHS · AI32591 · United States
External Links
PubMed source DOI: 10.1128/JVI.71.1.824-827.1997 PMC Full Text View journal details
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