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PMID: 10581267 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcriptional activation in yeast cells lacking transcription factor IIA.

Genetics ·Vol. 153 ·No. 4 ·1999-12-00 ·Pages 1573-81

Chou S, Chatterjee S, Lee M, Struhl K

Abstract

The general transcription factor IIA (TFIIA) forms a complex with TFIID at the TATA promoter element, and it inhibits the function of several negative regulators of the TATA-binding protein (TBP) subunit of TFIID. Biochemical experiments suggest that TFIIA is important in the response to transcriptional activators because activation domains can interact with TFIIA, increase recruitment of TFIID and TFIIA to the promoter, and promote isomerization of the TFIID-TFIIA-TATA complex. Here, we describe a double-shut-off approach to deplete yeast cells of Toa1, the large subunit of TFIIA, to <1% of the wild-type level. Interestingly, such TFIIA-depleted cells are essentially unaffected for activation by heat shock factor, Ace1, and Gal4-VP16. However, depletion of TFIIA causes a general two- to threefold decrease of transcription from most yeast promoters and a specific cell-cycle arrest at the G2-M boundary. These results indicate that transcriptional activation in vivo can occur in the absence of TFIIA.

MeSH Terms
Cell Cycle/genetics Promoter Regions, Genetic RNA Polymerase III/genetics Saccharomyces cerevisiae/cytology,genetics Saccharomyces cerevisiae Proteins Transcription Factor TFIIA Transcription Factors/genetics,metabolism Transcriptional Activation
Chemicals
Saccharomyces cerevisiae Proteins TOA1 protein, S cerevisiae Transcription Factor TFIIA Transcription Factors RNA Polymerase III
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chou S
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Chatterjee S
Lee M
Struhl K
References (50)
50 references, click to expand
  1. Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein.
    Mol Cell Biol. 1987 Jul;7(7):2316-28 PMID: 3302672
  2. Assembly of the isomerized TFIIA--TFIID--TATA ternary complex is necessary and sufficient for gene activation.
    Genes Dev. 1996 Oct 15;10(20):2540-50 PMID: 8895656
  3. The TATA-binding protein is required for transcription by all three nuclear RNA polymerases in yeast cells.
    Cell. 1992 May 15;69(4):685-96 PMID: 1586947
  4. Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis.
    Mol Cell Biol. 1987 May;7(5):1602-11 PMID: 3299050
  5. The high mobility group protein HMG1 can reversibly inhibit class II gene transcription by interaction with the TATA-binding protein.
    J Biol Chem. 1994 Jun 24;269(25):17136-40 PMID: 8006019
  6. NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization.
    Genes Dev. 1994 Mar 1;8(5):525-37 PMID: 7926748
  7. A mechanism for TAFs in transcriptional activation: activation domain enhancement of TFIID-TFIIA--promoter DNA complex formation.
    Genes Dev. 1994 May 1;8(9):995-1006 PMID: 7926793
  8. Mot1, a global repressor of RNA polymerase II transcription, inhibits TBP binding to DNA by an ATP-dependent mechanism.
    Genes Dev. 1994 Aug 15;8(16):1920-34 PMID: 7958867
  9. Drosophila TFIIA directs cooperative DNA binding with TBP and mediates transcriptional activation.
    Genes Dev. 1994 Oct 1;8(19):2313-23 PMID: 7958898
  10. Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription.
    Genes Dev. 1994 Oct 1;8(19):2324-35 PMID: 7958899
  11. Heat shock transcription factor activates yeast metallothionein gene expression in response to heat and glucose starvation via distinct signalling pathways.
    Mol Cell Biol. 1994 Dec;14(12):8155-65 PMID: 7969152
  12. Analysis of the yeast transcription factor TFIIA: distinct functional regions and a polymerase II-specific role in basal and activated transcription.
    Mol Cell Biol. 1995 Mar;15(3):1234-43 PMID: 7862117
  13. General requirement for RNA polymerase II holoenzymes in vivo.
    Proc Natl Acad Sci U S A. 1995 May 9;92(10):4587-90 PMID: 7753848
  14. KIN28 encodes a C-terminal domain kinase that controls mRNA transcription in Saccharomyces cerevisiae but lacks cyclin-dependent kinase-activating kinase (CAK) activity.
    Mol Cell Biol. 1995 Jun;15(6):2983-92 PMID: 7760796
  15. The KIN28 gene is required both for RNA polymerase II mediated transcription and phosphorylation of the Rpb1p CTD.
    J Mol Biol. 1995 Jun 9;249(3):535-44 PMID: 7783209
  16. Activation of the TFIID-TFIIA complex with HMG-2.
    Genes Dev. 1995 Jun 1;9(11):1354-65 PMID: 7797075
  17. The TBP-TFIIA interaction in the response to acidic activators in vivo.
    Science. 1995 Jul 7;269(5220):75-8 PMID: 7604282
  18. Mutations on the DNA-binding surface of TATA-binding protein can specifically impair the response to acidic activators in vivo.
    Mol Cell Biol. 1995 Oct;15(10):5461-9 PMID: 7565697
  19. A class of activation domains interacts directly with TFIIA and stimulates TFIIA-TFIID-promoter complex assembly.
    Mol Cell Biol. 1995 Nov;15(11):6465-73 PMID: 7565798
  20. Mechanism of differential utilization of the his3 TR and TC TATA elements.
    Mol Cell Biol. 1995 Dec;15(12):7059-66 PMID: 8524273
  21. Requirement of a corepressor for Dr1-mediated repression of transcription.
    Genes Dev. 1996 Apr 15;10(8):1033-48 PMID: 8608938
  22. Crystal structure of a yeast TFIIA/TBP/DNA complex.
    Nature. 1996 May 9;381(6578):127-51 PMID: 8610010
  23. Crystal structure of the yeast TFIIA/TBP/DNA complex.
    Science. 1996 May 10;272(5263):830-6 PMID: 8629014
  24. Separation of the transcriptional coactivator and antirepression functions of transcription factor IIA.
    Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6583-8 PMID: 8692860
  25. A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains.
    EMBO J. 1996 Jun 17;15(12):3105-16 PMID: 8670811
  26. Topology and reorganization of a human TFIID-promoter complex.
    Nature. 1996 Aug 22;382(6593):735-8 PMID: 8751448
  27. Interaction of the human T-cell lymphotropic virus type 1 tax transactivator with transcription factor IIA.
    Mol Cell Biol. 1996 Sep;16(9):4656-64 PMID: 8756622
  28. Transcription activation in cells lacking TAFIIS.
    Nature. 1996 Sep 12;383(6596):185-8 PMID: 8774886
  29. TBP-associated factors are not generally required for transcriptional activation in yeast.
    Nature. 1996 Sep 12;383(6596):188-91 PMID: 8774887
  30. Yeast TAF(II)90 is required for cell-cycle progression through G2/M but not for general transcription activation.
    Genes Dev. 1996 Sep 15;10(18):2368-80 PMID: 8824595
  31. Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo.
    Genes Dev. 1996 Oct 1;10(19):2491-504 PMID: 8843200
  32. High-resolution mapping of nucleoprotein complexes by site-specific protein-DNA photocrosslinking: organization of the human TBP-TFIIA-TFIIB-DNA quaternary complex.
    Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10620-5 PMID: 8855228
  33. The role of general initiation factors in transcription by RNA polymerase II.
    Trends Biochem Sci. 1996 Sep;21(9):327-35 PMID: 8870495
  34. The general transcription factors of RNA polymerase II.
    Genes Dev. 1996 Nov 1;10(21):2657-83 PMID: 8946909
  35. Evidence that Spt3 functionally interacts with Mot1, TFIIA, and TATA-binding protein to confer promoter-specific transcriptional control in Saccharomyces cerevisiae.
    Mol Cell Biol. 1997 Jan;17(1):287-95 PMID: 8972209
  36. The Dr1/DRAP1 heterodimer is a global repressor of transcription in vivo.
    Proc Natl Acad Sci U S A. 1997 Feb 4;94(3):820-5 PMID: 9023340
  37. A severely defective TATA-binding protein-TFIIB interaction does not preclude transcriptional activation in vivo.
    Mol Cell Biol. 1997 Mar;17(3):1336-45 PMID: 9032260
  38. Functional antagonism between RNA polymerase II holoenzyme and global negative regulator NC2 in vivo.
    Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3145-50 PMID: 9096360
  39. Yeast TAF(II)145 required for transcription of G1/S cyclin genes and regulated by the cellular growth state.
    Cell. 1997 Aug 22;90(4):607-14 PMID: 9288741
  40. Yeast TAF(II)145 functions as a core promoter selectivity factor, not a general coactivator.
    Cell. 1997 Aug 22;90(4):615-24 PMID: 9288742
  41. Mechanism of synergy between TATA and initiator: synergistic binding of TFIID following a putative TFIIA-induced isomerization.
    Genes Dev. 1997 Nov 15;11(22):3007-19 PMID: 9367983
  42. The yeast TAF145 inhibitory domain and TFIIA competitively bind to TATA-binding protein.
    Mol Cell Biol. 1998 Feb;18(2):1003-12 PMID: 9447997
  43. Cloning and biochemical characterization of TAF-172, a human homolog of yeast Mot1.
    Mol Cell Biol. 1998 Mar;18(3):1701-10 PMID: 9488487
  44. Association of transcription factor IIA with TATA binding protein is required for transcriptional activation of a subset of promoters and cell cycle progression in Saccharomyces cerevisiae.
    Mol Cell Biol. 1998 May;18(5):2559-70 PMID: 9566876
  45. Transcription factor IIA derepresses TATA-binding protein (TBP)-associated factor inhibition of TBP-DNA binding.
    J Biol Chem. 1998 Jun 5;273(23):14293-300 PMID: 9603936
  46. Simian virus 40 large T antigen stabilizes the TATA-binding protein-TFIIA complex on the TATA element.
    Mol Cell Biol. 1998 Jul;18(7):3926-35 PMID: 9632777
  47. DA-complex assembly activity required for VP16C transcriptional activation.
    Mol Cell Biol. 1998 Jul;18(7):4023-31 PMID: 9632787
  48. An activator target in the RNA polymerase II holoenzyme.
    Mol Cell. 1998 May;1(6):895-904 PMID: 9660972
  49. The histone H3-like TAF is broadly required for transcription in yeast.
    Mol Cell. 1998 Nov;2(5):675-82 PMID: 9844639
  50. Saturation mutagenesis of a yeast his3 "TATA element": genetic evidence for a specific TATA-binding protein.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2691-5 PMID: 3282236
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/153.4.1573
Published
1999-12-00
Pages
1573-81
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460864
Subset
IM
Grants
NIGMS NIH HHS · GM-30186 · United States
External Links
PubMed source DOI: 10.1093/genetics/153.4.1573 PMC Full Text View journal details
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