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PMID: 9632787 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DA-complex assembly activity required for VP16C transcriptional activation.

Molecular and cellular biology ·Vol. 18 ·No. 7 ·1998-07-00 ·Pages 4023-31

Kobayashi N, Horn PJ, Sullivan SM, Triezenberg SJ, Boyer TG, Berk AJ

Abstract

One class of transcriptional activation domains stimulates the concerted binding of TFIIA and TFIID to promoter DNA. To test whether this DA-complex assembly activity contributes significantly to the overall mechanism of activation in vivo, we analyzed mutants of the 38-amino-acid residue VP16C activation subdomain from herpes simplex virus. An excellent correlation was observed between the in vivo activation function of these mutants and their in vitro DA-complex assembly activity. Mutants severely defective for in vivo activation also showed reduced in vitro binding to native TFIIA. No significant correlation between in vivo activation function and in vitro binding to human TATA binding protein, human TFIIB, or Drosophila melanogaster TAFII40 was observed for this set of VP16C mutants. These results argue that the ability of VP16C to increase the rate and extent of DA-complex assembly makes a significant contribution to the overall mechanism of transcriptional activation in vivo.

MeSH Terms
Animals COS Cells DNA-Binding Proteins/genetics,metabolism HeLa Cells Herpes Simplex Virus Protein Vmw65/genetics,metabolism Humans Mutagenesis Peptides/metabolism Recombinant Fusion Proteins/genetics,metabolism TATA-Box Binding Protein Transcription Factor TFIIA Transcription Factor TFIID Transcription Factor TFIIH Transcription Factors/genetics,metabolism Transcription Factors, TFII/genetics,metabolism Transcriptional Activation
Chemicals
DNA-Binding Proteins Herpes Simplex Virus Protein Vmw65 Peptides Recombinant Fusion Proteins TATA-Box Binding Protein Transcription Factor TFIIA Transcription Factor TFIID Transcription Factors Transcription Factors, TFII Transcription Factor TFIIH
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kobayashi N
Department of Microbiology and Molecular Genetics, Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095-1570, USA.
Horn P J
Sullivan S M
Triezenberg S J
Boyer T G
Berk A J
References (58)
58 references, click to expand
  1. The acidic activator GAL4-AH can stimulate polymerase II transcription by promoting assembly of a closed complex requiring TFIID and TFIIA.
    Genes Dev. 1992 Sep;6(9):1716-27 PMID: 1516830
  2. Genetic isolation of ADA2: a potential transcriptional adaptor required for function of certain acidic activation domains.
    Cell. 1992 Jul 24;70(2):251-65 PMID: 1638630
  3. Pattern of aromatic and hydrophobic amino acids critical for one of two subdomains of the VP16 transcriptional activator.
    Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):883-7 PMID: 8381535
  4. Interaction between an acidic activator and transcription factor TFIIB is required for transcriptional activation.
    Nature. 1993 Jun 24;363(6431):741-4 PMID: 8515819
  5. Functional interaction of adenovirus E1A with holo-TFIID.
    Genes Dev. 1993 Sep;7(9):1810-23 PMID: 8370528
  6. The ZEBRA activation domain: modular organization and mechanism of action.
    Mol Cell Biol. 1993 Nov;13(11):7045-55 PMID: 8413294
  7. Residues in the TATA-binding protein required to mediate a transcriptional response to retinoic acid in EC cells.
    Nature. 1993 Oct 7;365(6446):562-6 PMID: 8413615
  8. Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB.
    Cell. 1993 Nov 5;75(3):519-30 PMID: 8221891
  9. An RNA polymerase II holoenzyme responsive to activators.
    Nature. 1994 Mar 31;368(6470):466-9 PMID: 8133894
  10. Transcriptional activation: a complex puzzle with few easy pieces.
    Cell. 1994 Apr 8;77(1):5-8 PMID: 8156597
  11. A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II.
    Cell. 1994 May 20;77(4):599-608 PMID: 8187178
  12. A mechanism for TAFs in transcriptional activation: activation domain enhancement of TFIID-TFIIA--promoter DNA complex formation.
    Genes Dev. 1994 May 1;8(9):995-1006 PMID: 7926793
  13. Binding of basal transcription factor TFIIH to the acidic activation domains of VP16 and p53.
    Mol Cell Biol. 1994 Oct;14(10):7013-24 PMID: 7935417
  14. Herpes simplex genes: the blueprint of a successful human pathogen.
    Trends Genet. 1994 Aug;10(8):267-74 PMID: 7940755
  15. Mot1, a global repressor of RNA polymerase II transcription, inhibits TBP binding to DNA by an ATP-dependent mechanism.
    Genes Dev. 1994 Aug 15;8(16):1920-34 PMID: 7958867
  16. Drosophila TFIIA directs cooperative DNA binding with TBP and mediates transcriptional activation.
    Genes Dev. 1994 Oct 1;8(19):2313-23 PMID: 7958898
  17. Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription.
    Genes Dev. 1994 Oct 1;8(19):2324-35 PMID: 7958899
  18. Reconstitution of human TFIIA activity from recombinant polypeptides: a role in TFIID-mediated transcription.
    Genes Dev. 1994 Oct 1;8(19):2336-48 PMID: 7958900
  19. Assembly of the isomerized TFIIA--TFIID--TATA ternary complex is necessary and sufficient for gene activation.
    Genes Dev. 1996 Oct 15;10(20):2540-50 PMID: 8895656
  20. Characterization of the basal inhibitor of class II transcription NC2 from Saccharomyces cerevisiae.
    Nucleic Acids Res. 1996 Nov 15;24(22):4450-5 PMID: 8948634
  21. The Dr1/DRAP1 heterodimer is a global repressor of transcription in vivo.
    Proc Natl Acad Sci U S A. 1997 Feb 4;94(3):820-5 PMID: 9023340
  22. A severely defective TATA-binding protein-TFIIB interaction does not preclude transcriptional activation in vivo.
    Mol Cell Biol. 1997 Mar;17(3):1336-45 PMID: 9032260
  23. Functional antagonism between RNA polymerase II holoenzyme and global negative regulator NC2 in vivo.
    Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3145-50 PMID: 9096360
  24. Transcriptional activation by recruitment.
    Nature. 1997 Apr 10;386(6625):569-77 PMID: 9121580
  25. RNA polymerase II holoenzyme and transcriptional regulation.
    Curr Opin Cell Biol. 1997 Jun;9(3):310-9 PMID: 9159076
  26. Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complex.
    Genes Dev. 1997 Jul 1;11(13):1640-50 PMID: 9224714
  27. Induced alpha helix in the VP16 activation domain upon binding to a human TAF.
    Science. 1997 Aug 29;277(5330):1310-3 PMID: 9271577
  28. Requirement for transcription factor IIA (TFIIA)-TFIID recruitment by an activator depends on promoter structure and template competition.
    Mol Cell Biol. 1997 Nov;17(11):6624-32 PMID: 9343426
  29. Transcriptional activation by TFIIB mutants that are severely impaired in interaction with promoter DNA and acidic activation domains.
    Mol Cell Biol. 1997 Dec;17(12):6794-802 PMID: 9372910
  30. Solution structure of the KIX domain of CBP bound to the transactivation domain of CREB: a model for activator:coactivator interactions.
    Cell. 1997 Dec 12;91(6):741-52 PMID: 9413984
  31. Transcriptional activation. Tuning-up transcription.
    Curr Biol. 1995 Jan 1;5(1):43-6 PMID: 7697348
  32. TATA-binding protein residues implicated in a functional interplay between negative cofactor NC2 (Dr1) and general factors TFIIA and TFIIB.
    J Biol Chem. 1995 May 5;270(18):10976-81 PMID: 7738039
  33. Association of an activator with an RNA polymerase II holoenzyme.
    Genes Dev. 1995 Apr 15;9(8):897-910 PMID: 7774808
  34. Molecular cloning and expression of the 32-kDa subunit of human TFIID reveals interactions with VP16 and TFIIB that mediate transcriptional activation.
    Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):5788-92 PMID: 7597030
  35. The TBP-TFIIA interaction in the response to acidic activators in vivo.
    Science. 1995 Jul 7;269(5220):75-8 PMID: 7604282
  36. Structure and function of transcriptional activation domains.
    Curr Opin Genet Dev. 1995 Apr;5(2):190-6 PMID: 7613088
  37. Characterization of physical interactions of the putative transcriptional adaptor, ADA2, with acidic activation domains and TATA-binding protein.
    J Biol Chem. 1995 Aug 18;270(33):19337-44 PMID: 7642611
  38. A general mechanism for transcriptional synergy by eukaryotic activators.
    Nature. 1995 Sep 21;377(6546):254-7 PMID: 7675113
  39. CCAAT/enhancer binding protein-alpha amino acid motifs with dual TBP and TFIIB binding ability co-operate to activate transcription in both yeast and mammalian cells.
    EMBO J. 1995 Sep 1;14(17):4318-28 PMID: 7556073
  40. A class of activation domains interacts directly with TFIIA and stimulates TFIIA-TFIID-promoter complex assembly.
    Mol Cell Biol. 1995 Nov;15(11):6465-73 PMID: 7565798
  41. The ability to associate with activation domains in vitro is not required for the TATA box-binding protein to support activated transcription in vivo.
    Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10550-4 PMID: 7479838
  42. Requirement of a corepressor for Dr1-mediated repression of transcription.
    Genes Dev. 1996 Apr 15;10(8):1033-48 PMID: 8608938
  43. Critical amino acids in the transcriptional activation domain of the herpesvirus protein VP16 are solvent-exposed in highly mobile protein segments. An intrinsic fluorescence study.
    J Biol Chem. 1996 Mar 1;271(9):4819-26 PMID: 8617751
  44. Quantitation of putative activator-target affinities predicts transcriptional activating potentials.
    EMBO J. 1996 Aug 1;15(15):3951-63 PMID: 8670900
  45. Mechanisms of transcriptional activation in vivo: two steps forward.
    Trends Genet. 1996 Aug;12(8):311-5 PMID: 8783941
  46. Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo.
    Genes Dev. 1996 Oct 1;10(19):2491-504 PMID: 8843200
  47. The role of general initiation factors in transcription by RNA polymerase II.
    Trends Biochem Sci. 1996 Sep;21(9):327-35 PMID: 8870495
  48. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  49. A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7410-4 PMID: 6760197
  50. Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma.
    Cell. 1984 Dec;39(3 Pt 2):653-62 PMID: 6096017
  51. GAL4 derivatives function alone and synergistically with mammalian activators in vitro.
    Cell. 1988 Aug 26;54(5):659-64 PMID: 3044607
  52. A small-scale procedure for preparation of nuclear extracts that support efficient transcription and pre-mRNA splicing.
    Gene Anal Tech. 1988 Mar-Apr;5(2):22-31 PMID: 3192155
  53. Five intermediate complexes in transcription initiation by RNA polymerase II.
    Cell. 1989 Feb 24;56(4):549-61 PMID: 2917366
  54. Transcription activation by the adenovirus E1a protein.
    Nature. 1989 Mar 2;338(6210):39-44 PMID: 2521923
  55. A vector for expressing GAL4(1-147) fusions in mammalian cells.
    Nucleic Acids Res. 1989 Sep 25;17(18):7539 PMID: 2798115
  56. Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID.
    Nature. 1990 Jun 28;345(6278):783-6 PMID: 2193231
  57. Selective inhibition of activated but not basal transcription by the acidic activation domain of VP16: evidence for transcriptional adaptors.
    Cell. 1990 Jun 29;61(7):1199-208 PMID: 2163758
  58. Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter.
    Genes Dev. 1992 Oct;6(10):1964-74 PMID: 1398073
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.7.4023
Published
1998-07-00
Pages
4023-31
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC108987
Subset
IM
Grants
NIAID NIH HHS · K04 AI01824 · United States
NIAID NIH HHS · AI27323 · United States
NCI NIH HHS · CA25235 · United States
External Links
PubMed source DOI: 10.1128/MCB.18.7.4023 PMC Full Text View journal details
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