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PMID: 1057177 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of human RNA transcripts among heterogeneous nuclear RNA from man-mouse somatic cell hybrids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 5 ·1975-05-00 ·Pages 1868-72

Di Cioccio RA, Voss R, Krim M, Grzeschik KH, Siniscalco M

Abstract

In man-mouse hybrid line from our cell library, the only cytological detectable portion of the human genome is the X chromosome, and the only genetic markers regularly expressed are coded by genes known to be ?X-linked. A component of the heterogeneous nuclear RNA of these cells was found to be complementary to repetitive human DNA sequences by means of RNA-DNA hybridization on nitrocellulose filters. The same procedure also permitted the identification of hybrid cell DNA sequences that are complementary to human heterogeneous nuclear RNA. This experimental approach, coupled with hybridization studies in situ, is expected to yield critical data on the distribution and the specificity of the repetitive DNA sequences present in the human genome and to provide a new tool for cytological mapping of human chromosomes.

MeSH Terms

Animals Cell Nucleus/metabolism Clone Cells Glucosephosphate Dehydrogenase/metabolism Humans Hybrid Cells/metabolism Hypoxanthine Phosphoribosyltransferase/metabolism Isocitrate Dehydrogenase/metabolism Karyotyping Malate Dehydrogenase/metabolism Mice Nucleic Acid Hybridization Phosphoglycerate Kinase/metabolism RNA/biosynthesis Sex Chromosomes/metabolism Transcription, Genetic

Chemicals

RNA Malate Dehydrogenase Isocitrate Dehydrogenase Glucosephosphate Dehydrogenase Hypoxanthine Phosphoribosyltransferase Phosphoglycerate Kinase

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Di Cioccio R A

Voss R

Krim M

Grzeschik K H

Siniscalco M

References (23)

23 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.72.5.1868

Published

1975-05-00

Pages

1868-72

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC432648

Subset

External Links

PubMed source DOI: 10.1073/pnas.72.5.1868 PMC Full Text View journal details

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Identification of human RNA transcripts among heterogeneous nuclear RNA from man-mouse somatic cell hybrids.

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