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PMID: 10562326 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Clnk, a novel SLP-76-related adaptor molecule expressed in cytokine-stimulated hemopoietic cells.

The Journal of experimental medicine ·Vol. 190 ·No. 10 ·1999-11-15 ·Pages 1527-34

Cao MY, Davidson D, Yu J, Latour S, Veillette A

Abstract

We have identified a novel Src homology 2 domain-containing leukocyte protein of 76 kD (SLP-76)-related molecule which we have termed Clnk (for cytokine-dependent hemopoietic cell linker). Unlike its relatives SLP-76 and B cell linker protein (Blnk), Clnk is not expressed uniformly within a given hemopoietic cell lineage. Even though it can be detected in several cell types, including T cells, natural killer cells, and mast cells, its expression seems to be strictly dependent on sustained exposure to cytokines such as interleukin (IL)-2 and IL-3. Strong support for the notion that Clnk is involved in immunoreceptor signaling was provided by the observation that it inducibly associated with at least one tyrosine-phosphorylated polypeptide (p92) in response to immunoreceptor stimulation. Moreover, transient expression of Clnk caused an increase in immunoreceptor-mediated signaling events in a T cell line. Taken together, these results show that Clnk is a novel member of the SLP-76 family selectively expressed in cytokine-stimulated hemopoietic cells. Furthermore, they suggest that Clnk may be involved in a cross-talk mechanism between cytokine receptor and immunoreceptor signaling.

MeSH Terms
Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Carrier Proteins/physiology Cell Line Cytokines/pharmacology DNA, Complementary/analysis DNA-Binding Proteins/metabolism Hematopoietic System/chemistry Mice Mice, Inbred C57BL Molecular Sequence Data NFATC Transcription Factors Nuclear Proteins Phosphoproteins/genetics,physiology Promoter Regions, Genetic Receptors, Immunologic/physiology Transcription Factor AP-1/metabolism Transcription Factors/metabolism src Homology Domains
Chemicals
Adaptor Proteins, Signal Transducing B cell linker protein Carrier Proteins Cytokines DNA, Complementary DNA-Binding Proteins NFATC Transcription Factors Nuclear Proteins Phosphoproteins Receptors, Immunologic SLP-76 signal Transducing adaptor proteins Transcription Factor AP-1 Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cao M Y
McGill Cancer Centre, McGill University, Montréal, Québec, Canada H3G 1Y6.
Davidson D
Yu J
Latour S
Veillette A
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.190.10.1527
Published
1999-11-15
Pages
1527-34
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2195705
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.190.10.1527 PMC Full Text View journal details
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