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PMID: 10557242 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The t-SNARE AtVAM3p resides on the prevacuolar compartment in Arabidopsis root cells.

Plant physiology ·Vol. 121 ·No. 3 ·1999-11-00 ·Pages 929-38

Sanderfoot AA, Kovaleva V, Zheng H, Raikhel NV

Abstract

Protein cargo is trafficked between the organelles of the endomembrane system inside transport vesicles, a process mediated by integral membrane proteins called SNAREs (soluble N-ethylmaleimide sensitive factor attachment protein receptors) that reside on the surface of the vesicle (v-SNAREs) and target membrane (t-SNAREs). In examining transport of cargo between the trans-Golgi network and the vacuole in Arabidopsis, we have previously characterized AtPEP12p as a t-SNARE residing on the prevacuolar compartment and AtVTI1a as a v-SNARE that interacts with AtPEP12p. Recently, we have begun to characterize AtVAM3p, another Arabidopsis t-SNARE that shows high sequence homology to AtPEP12p. We have found that AtVTI1a also interacts with AtVAM3p, suggesting a role for this t-SNARE in post-Golgi trafficking. AtVAM3p has been suggested to localize to the vacuolar membrane in Arabidopsis cells; however, using specific antisera and expression of epitope-tagged versions of each t-SNARE, we have discovered that AtVAM3p is found on the same prevacuolar structure as AtPEP12p in Arabidopsis root cells.

MeSH Terms
Arabidopsis/physiology,ultrastructure Arabidopsis Proteins Humans Intracellular Membranes/ultrastructure Membrane Proteins/analysis Phylogeny Plant Proteins/analysis,genetics Plant Roots/ultrastructure Qa-SNARE Proteins Vacuoles/physiology,ultrastructure
Chemicals
Arabidopsis Proteins Membrane Proteins Plant Proteins Qa-SNARE Proteins Vam3 protein, Arabidopsis
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sanderfoot A A
Michigan State University-Department of Energy Plant Research Laboratory, East Lansing, Michigan, 48824-1312, USA.
Kovaleva V
Zheng H
Raikhel N V
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.121.3.929
Published
1999-11-00
Pages
929-38
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC59457
Subset
IM
Grants
NIGMS NIH HHS · GM18861 · United States
External Links
PubMed source DOI: 10.1104/pp.121.3.929 PMC Full Text View journal details
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