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PMID: 9625693 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

An Arabidopsis VPS45p homolog implicated in protein transport to the vacuole.

Plant physiology ·Vol. 117 ·No. 2 ·1998-06-00 ·Pages 407-15

Bassham DC, Raikhel NV

Abstract

The Sec1p family of proteins is required for vesicle-mediated protein trafficking between various organelles of the endomembrane system. This family includes Vps45p, which is required for transport to the vacuole in yeast (Saccharomyces cerevisiae). We have isolated a cDNA encoding a VPS45 homolog from Arabidopsis thaliana (AtVPS45). The cDNA is able to complement both the temperature-sensitive growth defect and the vacuolar-targeting defect of a yeast vps45 mutant, indicating that the two proteins are functionally related. AtVPS45p is a peripheral membrane protein that associates with microsomal membranes. Sucrose-density gradient fractionation demonstrated that AtVPS45p co-fractionates with AtELP, a potential vacuolar protein sorting receptor, implying that they may reside on the same membrane populations. These results indicate that AtVPS45p is likely to function in the transport of proteins to the vacuole in plants.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,metabolism Arabidopsis Proteins Carrier Proteins/biosynthesis,chemistry,genetics Cloning, Molecular Humans Intracellular Membranes/metabolism Membrane Proteins/chemistry Microsomes/metabolism Molecular Sequence Data Plant Proteins/biosynthesis,chemistry,genetics Recombinant Proteins/biosynthesis,chemistry Saccharomyces cerevisiae/genetics,metabolism Sequence Alignment Sequence Homology, Amino Acid Vacuoles/metabolism Vesicular Transport Proteins
Chemicals
Arabidopsis Proteins Carrier Proteins Membrane Proteins Plant Proteins Recombinant Proteins VPS45 protein, Arabidopsis VPS45 protein, human Vesicular Transport Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bassham D C
Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824-1312, USA.
Raikhel N V
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.117.2.407
Published
1998-06-00
Pages
407-15
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC34960
Subset
IM
Databases
GENBANK
AF036234
External Links
PubMed source DOI: 10.1104/pp.117.2.407 PMC Full Text View journal details
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