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PMID: 10555775 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The impact of genetic removal of GFAP and/or vimentin on glutamine levels and transport of glucose and ascorbate in astrocytes.

Neurochemical research ·Vol. 24 ·No. 11 ·1999-11-00 ·Pages 1357-62

Pekny M, Eliasson C, Siushansian R, Ding M, Dixon SJ, Pekna M, Wilson JX, Hamberger A

Abstract

The importance of the intermediate filament (IF) proteins glial fibrillary acidic protein (GFAP) and vimentin for astrocyte function was studied by investigating astrocytes prepared from GFAP-/- and/or vimentin-/- mice. The rate of glucose uptake through facilitative hexose transporters was not affected by depletion of GFAP or vimentin. Similarly, the absence of these IF proteins did not affect ascorbate uptake, under control or cyclic AMP-stimulated conditions, or ascorbate efflux through volume-sensitive organic anion channels. However, compared with wild-type astrocytes, glutamine concentrations were increased up to 200% in GFAP-/- astrocytes and up to 150% in GFAP+/- astrocytes and this increase was not dependent on the presence of vimentin. GFAP-/- astrocytes in culture still contain IFs (made of vimentin and nestin), whereas GFAP-/- vim-/- cultured astrocytes lack IFs. Thus, glutamine levels appear to correlate inversely with GFAP, rather than depend on the presence of IFs per se. Furthermore, the effect of GFAP is dose-dependent since the glutamine concentration in GFAP+/- astrocytes falls between those in wild-type and GFAP-/- astrocytes.

MeSH Terms
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/pharmacology Animals Ascorbic Acid/metabolism Astrocytes/metabolism Biological Transport Bucladesine/pharmacology Cells, Cultured Cyclic AMP/pharmacology Glial Fibrillary Acidic Protein/deficiency,physiology Glucose/metabolism Glutamine/metabolism Ion Channels/physiology Mice Mice, Inbred C57BL Vimentin/deficiency,physiology
Chemicals
Glial Fibrillary Acidic Protein Ion Channels Vimentin Glutamine Bucladesine Cyclic AMP Glucose Ascorbic Acid 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Pekny M
Department of Medical Biochemistry, University of Gothenburg, Sweden. Milos.Pekny@medkem.gu.se
Eliasson C
Siushansian R
Ding M
Dixon S J
Pekna M
Wilson J X
Hamberger A
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Article Info
Journal
Neurochemical research
Abbr.
Neurochem Res
ISSN
0364-3190
DOI
10.1023/a:1022572304626
Email
milos.pekny@medkem.gu.se
Published
1999-11-00
Pages
1357-62
Language
English
Region
United States
NLM ID
7613461
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1022572304626 View journal details
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