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PMID: 10542158 Published · ppublish English Journal Article

Functional interactions of a homolog of proliferating cell nuclear antigen with DNA polymerases in Archaea.

Journal of bacteriology ·Vol. 181 ·No. 21 ·1999-11-00 ·Pages 6591-9

Cann IK, Ishino S, Hayashi I, Komori K, Toh H, Morikawa K, Ishino Y

Abstract

Proliferating cell nuclear antigen (PCNA) is an essential component of the DNA replication and repair machinery in the domain Eucarya. We cloned the gene encoding a PCNA homolog (PfuPCNA) from an euryarchaeote, Pyrococcus furiosus, expressed it in Escherichia coli, and characterized the biochemical properties of the gene product. The protein PfuPCNA stimulated the in vitro primer extension abilities of polymerase (Pol) I and Pol II, which are the two DNA polymerases identified in this organism to date. An immunological experiment showed that PfuPCNA interacts with both Pol I and Pol II. Pol I is a single polypeptide with a sequence similar to that of family B (alpha-like) DNA polymerases, while Pol II is a heterodimer. PfuPCNA interacted with DP2, the catalytic subunit of the heterodimeric complex. These results strongly support the idea that the PCNA homolog works as a sliding clamp of DNA polymerases in P. furiosus, and the basic mechanism for the processive DNA synthesis is conserved in the domains Bacteria, Eucarya, and Archaea. The stimulatory effect of PfuPCNA on the DNA synthesis was observed by using a circular DNA template without the clamp loader (replication factor C [RFC]) in both Pol I and Pol II reactions in contrast to the case of eukaryotic organisms, which are known to require the RFC to open the ring structure of PCNA prior to loading onto a circular DNA. Because RFC homologs have been found in the archaeal genomes, they may permit more efficient stimulation of DNA synthesis by archaeal DNA polymerases in the presence of PCNA. This is the first stage in elucidating the archaeal DNA replication mechanism.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Polymerase I/metabolism DNA Polymerase II/metabolism DNA, Archaeal/chemistry,genetics DNA-Directed DNA Polymerase/metabolism Eukaryotic Cells/metabolism Genes, Archaeal Humans Molecular Sequence Data Proliferating Cell Nuclear Antigen/chemistry,genetics,metabolism Pyrococcus furiosus/genetics,metabolism Recombinant Proteins/isolation & purification,metabolism Sequence Homology, Amino Acid
Chemicals
DNA, Archaeal Proliferating Cell Nuclear Antigen Recombinant Proteins DNA Polymerase I DNA Polymerase II DNA-Directed DNA Polymerase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Cann I K
Department of Molecular Biology, Biomolecular Engineering Research Institute, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan.
Ishino S
Hayashi I
Komori K
Toh H
Morikawa K
Ishino Y
References (45)
45 references, click to expand
  1. Expression and physicochemical characterization of human proliferating cell nuclear antigen.
    Biochemistry. 1995 Aug 29;34(34):10703-12 PMID: 7662654
  2. Production of active mouse DNA polymerase delta in bacteria.
    Gene. 1995 Jun 9;158(2):241-6 PMID: 7607549
  3. Schizosaccharomyces pombe proliferating cell nuclear antigen mutations affect DNA polymerase delta processivity.
    J Biol Chem. 1996 Jul 5;271(27):15971-80 PMID: 8663159
  4. Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.
    Science. 1996 Aug 23;273(5278):1058-73 PMID: 8688087
  5. A non-alpha-like DNA polymerase from the hyperthermophilic archaeon Pyrococcus furiosus.
    Biol Pharm Bull. 1995 Dec;18(12):1647-52 PMID: 8787781
  6. Thermostable DNA polymerases.
    Adv Protein Chem. 1996;48:377-435 PMID: 8791630
  7. Structure of the C-terminal region of p21(WAF1/CIP1) complexed with human PCNA.
    Cell. 1996 Oct 18;87(2):297-306 PMID: 8861913
  8. Purification and characterization of the catalytic subunit of human DNA polymerase delta expressed in baculovirus-infected insect cells.
    J Biol Chem. 1996 Nov 22;271(47):29740-5 PMID: 8939909
  9. PCNA: structure, functions and interactions.
    Oncogene. 1997 Feb 13;14(6):629-40 PMID: 9038370
  10. The small subunit is required for functional interaction of DNA polymerase delta with the proliferating cell nuclear antigen.
    Nucleic Acids Res. 1997 Mar 15;25(6):1094-9 PMID: 9092615
  11. Expression and characterization of the small subunit of human DNA polymerase delta.
    J Biol Chem. 1997 May 16;272(20):13013-8 PMID: 9148910
  12. Homologous regions of Fen1 and p21Cip1 compete for binding to the same site on PCNA: a potential mechanism to co-ordinate DNA replication and repair.
    Oncogene. 1997 May 15;14(19):2313-21 PMID: 9178907
  13. Mutations in yeast proliferating cell nuclear antigen define distinct sites for interaction with DNA polymerase delta and DNA polymerase epsilon.
    Mol Cell Biol. 1997 Nov;17(11):6367-78 PMID: 9343398
  14. A novel DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus: gene cloning, expression, and characterization.
    Genes Cells. 1997 Aug;2(8):499-512 PMID: 9348040
  15. Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics.
    J Bacteriol. 1997 Nov;179(22):7135-55 PMID: 9371463
  16. The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus.
    Nature. 1997 Nov 27;390(6658):364-70 PMID: 9389475
  17. Proliferating cell nuclear antigen: more than a clamp for DNA polymerases.
    Bioessays. 1997 Nov;19(11):967-75 PMID: 9394619
  18. A tRNA(Glu) gene from the hyperthermophilic archaeon Pyrococcus furiosus contains the 3'-terminal CCA sequence of the mature tRNA.
    FEMS Microbiol Lett. 1998 Mar 15;160(2):199-204 PMID: 9532738
  19. A novel DNA polymerase family found in Archaea.
    J Bacteriol. 1998 Apr;180(8):2232-6 PMID: 9555910
  20. PCNA binding through a conserved motif.
    Bioessays. 1998 Mar;20(3):195-9 PMID: 9631646
  21. Characterization of the two small subunits of Saccharomyces cerevisiae DNA polymerase delta.
    J Biol Chem. 1998 Jul 31;273(31):19747-55 PMID: 9677405
  22. Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3.
    DNA Res. 1998 Apr 30;5(2):55-76 PMID: 9679194
  23. Protein-PCNA interactions: a DNA-scanning mechanism?
    Trends Biochem Sci. 1998 Jul;23(7):236-8 PMID: 9697409
  24. The DNA replication fork in eukaryotic cells.
    Annu Rev Biochem. 1998;67:721-51 PMID: 9759502
  25. A heterodimeric DNA polymerase: evidence that members of Euryarchaeota possess a distinct DNA polymerase.
    Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14250-5 PMID: 9826686
  26. PCNA, a multifunctional ring on DNA.
    Biochim Biophys Acta. 1998 Nov 26;1443(1-2):23-39 PMID: 9838028
  27. The euryarchaeotes, a subdomain of Archaea, survive on a single DNA polymerase: fact or farce?
    Genes Genet Syst. 1998 Dec;73(6):323-36 PMID: 10333564
  28. Phylogenetic structure of the prokaryotic domain: the primary kingdoms.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):5088-90 PMID: 270744
  29. Archaeal DNA replication: identifying the pieces to solve a puzzle.
    Genetics. 1999 Aug;152(4):1249-67 PMID: 10430556
  30. Two family B DNA polymerases from Aeropyrum pernix, an aerobic hyperthermophilic crenarchaeote.
    J Bacteriol. 1999 Oct;181(19):5984-92 PMID: 10498710
  31. Aphidicolin inhibits growth and DNA synthesis in halophilic arachaebacteria.
    J Bacteriol. 1984 Aug;159(2):800-2 PMID: 6204969
  32. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  33. Structural and enzymatic studies of the T4 DNA replication system. I. Physical characterization of the polymerase accessory protein complex.
    J Biol Chem. 1989 Jul 25;264(21):12709-16 PMID: 2663867
  34. Calculation of protein extinction coefficients from amino acid sequence data.
    Anal Biochem. 1989 Nov 1;182(2):319-26 PMID: 2610349
  35. Two forms of DNA polymerase delta from mouse cells. Purification and properties.
    J Biol Chem. 1990 Sep 25;265(27):16402-11 PMID: 1975812
  36. Three-dimensional structure of the beta subunit of E. coli DNA polymerase III holoenzyme: a sliding DNA clamp.
    Cell. 1992 May 1;69(3):425-37 PMID: 1349852
  37. Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities.
    J Biol Chem. 1993 Jan 25;268(3):1965-75 PMID: 8420970
  38. Organization and nucleotide sequence of the DNA polymerase gene from the archaeon Pyrococcus furiosus.
    Nucleic Acids Res. 1993 Jan 25;21(2):259-65 PMID: 8441634
  39. Interaction of proliferating cell nuclear antigen with yeast DNA polymerase delta.
    J Biol Chem. 1993 Oct 15;268(29):21706-10 PMID: 8104944
  40. Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA.
    Cell. 1994 Dec 30;79(7):1233-43 PMID: 8001157
  41. DNA polymerase delta holoenzyme: action on single-stranded DNA and on double-stranded DNA in the presence of replicative DNA helicases.
    Biochemistry. 1995 Apr 18;34(15):5003-10 PMID: 7711022
  42. A conserved region in the amino terminus of DNA polymerase delta is involved in proliferating cell nuclear antigen binding.
    J Biol Chem. 1995 Apr 7;270(14):7988-92 PMID: 7713898
  43. Expression of the catalytic subunit of human DNA polymerase delta in mammalian cells using a vaccinia virus vector system.
    J Biol Chem. 1995 Apr 7;270(14):7993-8 PMID: 7713899
  44. The hyperthermophilic archaeon Pyrodictium occultum has two alpha-like DNA polymerases.
    J Bacteriol. 1995 Apr;177(8):2164-77 PMID: 7721707
  45. DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine.
    Annu Rev Biochem. 1995;64:171-200 PMID: 7574479
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.21.6591-6599.1999
Published
1999-11-00
Pages
6591-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94121
Subset
IM
Databases
GENBANK
AB017486
External Links
PubMed source DOI: 10.1128/JB.181.21.6591-6599.1999 PMC Full Text View journal details
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