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PMID: 10535980 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

LFG: an anti-apoptotic gene that provides protection from Fas-mediated cell death.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 22 ·1999-10-26 ·Pages 12667-72

Somia NV, Schmitt MJ, Vetter DE, Van Antwerp D, Heinemann SF, Verma IM

Abstract

Programmed cell death regulates a number of biological phenomena, and the apoptotic signal must itself be tightly controlled to avoid inappropriate cell death. We established a genetic screen to search for molecules that inhibit the apoptotic signal from the Fas receptor. Here we report the isolation of a gene, LFG, that protects cells uniquely from Fas but not from the mechanistically related tumor necrosis factor alpha death signal. LFG is widely distributed, but remarkably is highly expressed in the hippocampus. LFG can bind to the Fas receptor, but does not regulate Fas expression or interfere with binding of an agonist antibody. Furthermore LFG does not inhibit binding of FADD to Fas.

MeSH Terms
Amino Acid Sequence Antigens, CD/physiology Apoptosis/genetics Apoptosis Regulatory Proteins Base Sequence Blotting, Western Carrier Proteins/genetics Cell Line Cloning, Molecular DNA Primers Down-Regulation Fluorescent Antibody Technique HeLa Cells Humans Membrane Proteins Molecular Sequence Data Precipitin Tests Receptors, Tumor Necrosis Factor/physiology Receptors, Tumor Necrosis Factor, Type I Sequence Homology, Amino Acid fas Receptor/physiology
Chemicals
Antigens, CD Apoptosis Regulatory Proteins Carrier Proteins DNA Primers FAIM2 protein, human Membrane Proteins Receptors, Tumor Necrosis Factor Receptors, Tumor Necrosis Factor, Type I fas Receptor
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Somia N V
The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Schmitt M J
Vetter D E
Van Antwerp D
Heinemann S F
Verma I M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.22.12667
Published
1999-10-26
Pages
12667-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC23041
Subset
IM
Grants
NIA NIH HHS · AG10435 · United States
NCI NIH HHS · CA44360 · United States
NIA NIH HHS · P01 AG010435 · United States
NHLBI NIH HHS · R01 HL053670 · United States
NHLBI NIH HHS · HL3670 · United States
External Links
PubMed source DOI: 10.1073/pnas.96.22.12667 PMC Full Text View journal details
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