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PMID: 9228018 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

FLAME-1, a novel FADD-like anti-apoptotic molecule that regulates Fas/TNFR1-induced apoptosis.

The Journal of biological chemistry ·Vol. 272 ·No. 30 ·1997-07-25 ·Pages 18542-5

Srinivasula SM, Ahmad M, Ottilie S, Bullrich F, Banks S, Wang Y, Fernandes-Alnemri T, Croce CM, Litwack G, Tomaselli KJ, Armstrong RC, Alnemri ES

Abstract

We identified and cloned a novel human protein that contains FADD/Mort1 death effector domain homology regions, designated FLAME-1. FLAME-1, although most similar in structure to Mch4 and Mch5, does not possess caspase activity but can interact specifically with FADD, Mch4, and Mch5. Interestingly, FLAME-1 is recruited to the Fas receptor complex and can abrogate Fas/TNFR-induced apoptosis upon expression in FasL/tumor necrosis factor-sensitive MCF-7 cells, possibly by acting as a dominant-negative inhibitor. These findings identify a novel endogenous control point that regulates Fas/TNFR1-mediated apoptosis.

MeSH Terms
Adaptor Proteins, Signal Transducing Amino Acid Sequence Apoptosis/drug effects,radiation effects CASP8 and FADD-Like Apoptosis Regulating Protein Carrier Proteins/chemistry,genetics,metabolism Caspase 10 Caspase 8 Caspase 9 Caspases Cloning, Molecular Cysteine Endopeptidases/chemistry,metabolism Fas Ligand Protein Fas-Associated Death Domain Protein Humans Intracellular Signaling Peptides and Proteins Membrane Glycoproteins/metabolism Molecular Sequence Data Proteins/metabolism Sequence Homology, Amino Acid TNF Receptor-Associated Factor 1 Tissue Distribution Ultraviolet Rays
Chemicals
Adaptor Proteins, Signal Transducing CASP8 and FADD-Like Apoptosis Regulating Protein CFLAR protein, human Carrier Proteins FADD protein, human FASLG protein, human Fas Ligand Protein Fas-Associated Death Domain Protein Intracellular Signaling Peptides and Proteins Membrane Glycoproteins Proteins TNF Receptor-Associated Factor 1 CASP8 protein, human CASP9 protein, human Caspase 10 Caspase 8 Caspase 9 Caspases Cysteine Endopeptidases CASP10 protein, human
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Srinivasula S M
Center for Apoptosis Research and the Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Ahmad M
Ottilie S
Bullrich F
Banks S
Wang Y
Fernandes-Alnemri T
Croce C M
Litwack G
Tomaselli K J
Armstrong R C
Alnemri E S
Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
Published
1997-07-25
Pages
18542-5
Language
English
Region
United States
NLM ID
2985121R
Subset
IM
Grants
NIA NIH HHS · AG 13487 · United States
Databases
GENBANK
AF009616, AF009617, AF009618, AF009619, AF009620
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