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PMID: 10531204 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of pic, a secreted protease of Shigella flexneri and enteroaggregative Escherichia coli.

Infection and immunity ·Vol. 67 ·No. 11 ·1999-11-00 ·Pages 5587-96

Henderson IR, Czeczulin J, Eslava C, Noriega F, Nataro JP

Abstract

We have identified and characterized a secreted protein, designated Pic, which is encoded on the chromosomes of enteroaggregative Escherichia coli (EAEC) 042 and Shigella flexneri 2457T. The product of the pic gene is synthesized as a 146.5-kDa precursor molecule which is processed at the N and C termini during secretion, allowing the release of a mature protein (109.8 kDa) into the culture supernatant. The deduced amino acid sequence of Pic shows high homology to autotransporter proteins, particularly a subgroup termed the SPATEs (serine protease autotransporters of the Enterobacteriaceae). Present in all members of this subgroup is a motif similar to the active sites of certain serine proteases. Pic catalyzes gelatin degradation, which can be abolished by disruption of the predicted proteolytic active site. Functional analysis of the Pic protein implicates this factor in mucinase activity, serum resistance, and hemagglutination. Our data suggest that Pic may be a multifunctional protein involved in enteric pathogenesis.

MeSH Terms
Adult Animals Bacterial Proteins/chemistry,genetics,metabolism Blood Bactericidal Activity Cloning, Molecular Endopeptidases/chemistry,genetics,metabolism Escherichia coli/enzymology,pathogenicity Hemagglutination Humans Mice Molecular Sequence Data Rabbits Serine Proteases/chemistry,genetics,metabolism Shigella flexneri/enzymology,pathogenicity
Chemicals
Bacterial Proteins Endopeptidases Pic protein, Shigella flexneri Serine Proteases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Henderson I R
Center for Vaccine Development, Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA. ihenders@umaryland.edu
Czeczulin J
Eslava C
Noriega F
Nataro J P
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.67.11.5587-5596.1999
Email
ihenders@umaryland.edu
Published
1999-11-00
Pages
5587-96
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC96930
Subset
IM
Grants
NIAID NIH HHS · R01 AI033096 · United States
NIAID NIH HHS · AI33096 · United States
Databases
GENBANK
AF097644
External Links
PubMed source DOI: 10.1128/IAI.67.11.5587-5596.1999 PMC Full Text View journal details
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