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PMID: 10515997 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

An Mpsi-containing heterologous RNA, but not env mRNA, is efficiently packaged into avian retroviral particles.

Journal of virology ·Vol. 73 ·No. 11 ·1999-11-00 ·Pages 8926-33

Banks JD, Kealoha BO, Linial ML

Abstract

Retroviruses preferentially package full-length genomic RNA over spliced viral messages. For most retroviruses, this preference is likely due to the absence of all or part of the packaging signal on subgenomic RNAs. In avian leukosis-sarcoma virus, however, we have shown that the minimal packaging signal, MPsi, is located upstream of the 5' splice site and therefore is present on both genomic and spliced RNAs. We now show that an MPsi-containing heterologous RNA is packaged only 2.6-fold less efficiently than genomic Rous sarcoma virus RNA. Thus, few additional packaging sequences and/or structures exist outside of MPsi. In contrast, we found that env mRNA is not efficiently packaged. These results indicate that either MPsi is not functional on this RNA or the RNA is somehow segregated from the packaging machinery. Finally, deletion of sequences from the 3' end of MPsi was found to reduce the packaging efficiency of heterologous RNAs.

MeSH Terms
Alpharetrovirus/metabolism Avian Sarcoma Viruses/metabolism Base Sequence Cell Line Gene Products, env/biosynthesis,genetics Molecular Sequence Data Nucleic Acid Conformation Plasmids RNA Splicing RNA, Messenger/chemistry,genetics,metabolism RNA, Viral/chemistry,genetics,metabolism Viral Proteins/analysis Virus Assembly
Chemicals
Gene Products, env RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Banks J D
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
Kealoha B O
Linial M L
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.73.11.8926-8933.1999
Published
1999-11-00
Pages
8926-33
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC112923
Subset
IM
Grants
NCI NIH HHS · R01 CA018282 · United States
NCI NIH HHS · CA 18282 · United States
External Links
PubMed source DOI: 10.1128/JVI.73.11.8926-8933.1999 PMC Full Text View journal details
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