Gene disruption studies of penicillin-binding proteins 1a, 1b, and 2a in Streptococcus pneumoniae.

Journal of bacteriology ·Vol. 181 ·No. 20 ·1999-10-00 ·Pages 6552-5

Hoskins J, Matsushima P, Mullen DL, Tang J, Zhao G, Meier TI, Nicas TI, Jaskunas SR

Abstract

The effects of inactivation of the genes encoding penicillin-binding protein 1a (PBP1a), PBP1b, and PBP2a in Streptococcus pneumoniae were examined. Insertional mutants did not exhibit detectable changes in growth rate or morphology, although a pbp1a pbp1b double-disruption mutant grew more slowly than its parent did. Attempts to generate a pbp1a pbp2a double-disruption mutant failed. The pbp2a mutants, but not the other mutants, were more sensitive to moenomycin, a transglycosylase inhibitor. These observations suggest that individually the pbp1a, pbp1b, and pbp2a genes are dispensable but that either pbp1a or pbp2a is required for growth in vitro. These results also suggest that PBP2a is a functional transglycosylase in S. pneumoniae.

MeSH Terms

Bacterial Proteins Bambermycins/pharmacology Carrier Proteins/genetics Genes, Bacterial Glycosyltransferases/antagonists & inhibitors Hexosyltransferases/genetics Microbial Sensitivity Tests Molecular Sequence Data Multienzyme Complexes/genetics Muramoylpentapeptide Carboxypeptidase/genetics Mutagenesis, Insertional Penicillin-Binding Proteins Peptide Synthases Peptidyl Transferases/genetics Streptococcus pneumoniae/genetics

Chemicals

Bacterial Proteins Carrier Proteins Multienzyme Complexes Penicillin-Binding Proteins penicillin-binding protein 2a, Streptococcus Bambermycins Peptidyl Transferases Glycosyltransferases Hexosyltransferases Muramoylpentapeptide Carboxypeptidase Peptide Synthases

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Hoskins J

Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, Indiana 46285, USA. Hoskins_JoAnn@Lilly.com

Matsushima P

Mullen D L

Tang J

Zhao G

Meier T I

Nicas T I

Jaskunas S R

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31 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/JB.181.20.6552-6555.1999

hoskins_joann@lilly.com

Published

1999-10-00

Pages

6552-5

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC103796

Subset

Databases

GENBANK

AF101780, AF101781

External Links

PubMed source DOI: 10.1128/JB.181.20.6552-6555.1999 PMC Full Text View journal details

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Gene disruption studies of penicillin-binding proteins 1a, 1b, and 2a in Streptococcus pneumoniae.

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