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PMID: 10498737 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interstrain variation of the polysaccharide B biosynthesis locus of Bacteroides fragilis: characterization of the region from strain 638R.

Journal of bacteriology ·Vol. 181 ·No. 19 ·1999-10-00 ·Pages 6192-6

Comstock LE, Coyne MJ, Tzianabos AO, Kasper DL

Abstract

The sequence and analysis of the capsular polysaccharide biosynthesis locus, PS B2, of Bacteroides fragilis 638R are described, and the sequence is compared with that of the PS B1 biosynthesis locus of B. fragilis NCTC 9343. Two genes of the region, wcgD and wcgC, are shown by complementation to encode a UDP-N-acetylglucosamine 2-epimerase and a UDP-N-acetylmannosamine dehydrogenase, respectively.

MeSH Terms
Bacterial Capsules/biosynthesis Bacteroides fragilis/enzymology,genetics Carbohydrate Dehydrogenases/genetics Carbohydrate Epimerases/genetics Escherichia coli Proteins Genes, Bacterial Genetic Complementation Test Molecular Sequence Data
Chemicals
Escherichia coli Proteins Carbohydrate Dehydrogenases UDP-N-acetylmannosamine dehydrogenase Carbohydrate Epimerases UDP acetylglucosamine-2-epimerase wecB protein, E coli
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Comstock L E
Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA. lcomstock@channing.harvard.edu
Coyne M J
Tzianabos A O
Kasper D L
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.19.6192-6196.1999
Email
lcomstock@channing.harvard.edu
Published
1999-10-00
Pages
6192-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC103652
Subset
IM
Grants
NIAID NIH HHS · AI39576 · United States
NIAID NIH HHS · R01 AI044193-01 · United States
NIAID NIH HHS · AI44193 · United States
NIAID NIH HHS · R56 AI044193 · United States
NIAID NIH HHS · R01 AI044193 · United States
NIAID NIH HHS · R01 AI039576 · United States
Databases
GENBANK
AF125164
External Links
PubMed source DOI: 10.1128/JB.181.19.6192-6196.1999 PMC Full Text View journal details
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