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PMID: 10488230 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The phosphoenolpyruvate/phosphate translocator is required for phenolic metabolism, palisade cell development, and plastid-dependent nuclear gene expression.

The Plant cell ·Vol. 11 ·No. 9 ·1999-09-00 ·Pages 1609-22

Streatfield SJ, Weber A, Kinsman EA, Häusler RE, Li J, Post-Beittenmiller D, Kaiser WM, Pyke KA, Flügge UI, Chory J

Abstract

The Arabidopsis chlorophyll a/b binding protein (CAB) gene underexpressed 1 (cue1) mutant underexpresses light-regulated nuclear genes encoding chloroplast-localized proteins. cue1 also exhibits mesophyll-specific chloroplast and cellular defects, resulting in reticulate leaves. Both the gene underexpression and the leaf cell morphology phenotypes are dependent on light intensity. In this study, we determine that CUE1 encodes the plastid inner envelope phosphoenolpyruvate/phosphate translocator (PPT) and define amino acid residues that are critical for translocator function. The biosynthesis of aromatics is compromised in cue1, and the reticulate phenotype can be rescued by feeding aromatic amino acids. Determining that CUE1 encodes PPT indicates the in vivo role of the translocator in metabolic partitioning and reveals a mesophyll cell-specific requirement for the translocator in Arabidopsis leaves. The nuclear gene expression defects in cue1 suggest that a light intensity-dependent interorganellar signal is modulated through metabolites dependent on a plastid supply of phosphoenolpyruvate.

MeSH Terms
Arabidopsis/cytology,genetics,metabolism Base Sequence Chlorophyll/biosynthesis Chlorophyll A DNA, Plant/genetics Gene Expression Genes, Plant Light Molecular Sequence Data Mutation Phenols/metabolism Phenotype Phosphates/metabolism Phosphoenolpyruvate/metabolism Photosynthesis Plant Proteins/metabolism Plastids/genetics Plastoquinone/metabolism Shikimic Acid/metabolism
Chemicals
DNA, Plant Phenols Phosphates Plant Proteins Chlorophyll Shikimic Acid Phosphoenolpyruvate Plastoquinone Chlorophyll A
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Streatfield S J
Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA.
Weber A
Kinsman E A
Häusler R E
Li J
Post-Beittenmiller D
Kaiser W M
Pyke K A
Flügge U I
Chory J
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.11.9.1609
Published
1999-09-00
Pages
1609-22
Language
English
Region
England
NLM ID
9208688
PMCID
PMC144315
Subset
IM
Corrections
CommentIn
PMID: 10488229
External Links
PubMed source DOI: 10.1105/tpc.11.9.1609 PMC Full Text View journal details
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