Home LiteratureArticle Details
PMID: 10471721 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genomic organization of the S locus: Identification and characterization of genes in SLG/SRK region of S(9) haplotype of Brassica campestris (syn. rapa).

Genetics ·Vol. 153 ·No. 1 ·1999-09-00 ·Pages 391-400

Suzuki G, Kai N, Hirose T, Fukui K, Nishio T, Takayama S, Isogai A, Watanabe M, Hinata K

Abstract

In Brassica, two self-incompatibility genes, encoding SLG (S locus glycoprotein) and SRK (S-receptor kinase), are located at the S locus and expressed in the stigma. Recent molecular analysis has revealed that the S locus is highly polymorphic and contains several genes, i.e., SLG, SRK, the as-yet-unidentified pollen S gene(s), and other linked genes. In the present study, we searched for expressed sequences in a 76-kb SLG/SRK region of the S(9) haplotype of Brassica campestris (syn. rapa) and identified 10 genes in addition to the four previously identified (SLG(9), SRK(9), SAE1, and SLL2) in this haplotype. This gene density (1 gene/5.4 kb) suggests that the S locus is embedded in a gene-rich region of the genome. The average G + C content in this region is 32.6%. An En/Spm-type transposon-like element was found downstream of SLG(9). Among the genes we identified that had not previously been found to be linked to the S locus were genes encoding a small cysteine-rich protein, a J-domain protein, and an antisilencing protein (ASF1) homologue. The small cysteine-rich protein was similar to a pollen coat protein, named PCP-A1, which had previously been shown to bind SLG.

MeSH Terms
Amino Acid Sequence Base Composition Brassica/enzymology,genetics Cloning, Molecular DNA Transposable Elements/genetics Gene Expression Gene Expression Regulation, Plant Gene Library Genes, Plant/genetics Genetic Linkage/genetics Genome, Plant Glycoproteins/genetics Haplotypes/genetics Molecular Sequence Data Open Reading Frames/genetics Physical Chromosome Mapping Plant Proteins/genetics Plant Structures/enzymology,genetics,growth & development Polymorphism, Genetic/genetics RNA, Messenger/analysis,genetics Sequence Homology, Amino Acid
Chemicals
DNA Transposable Elements Glycoproteins Plant Proteins RNA, Messenger self-incompatibility locus specific glycoprotein, Brassica
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Suzuki G
Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan. gsuzuki@bios.tohoku.ac.jp
Kai N
Hirose T
Fukui K
Nishio T
Takayama S
Isogai A
Watanabe M
Hinata K
References (36)
36 references, click to expand
  1. Visual verification of close disposition between a rice A genome-specific DNA sequence (TrsA) and the telomere sequence.
    Plant Mol Biol. 1997 Dec;35(6):963-8 PMID: 9426615
  2. PCP-A1, a defensin-like Brassica pollen coat protein that binds the S locus glycoprotein, is the product of gametophytic gene expression.
    Plant Cell. 1998 Aug;10(8):1333-47 PMID: 9707533
  3. Molecular characterization of the S locus in two self-incompatible Brassica napus lines.
    Plant Cell. 1996 Dec;8(12):2369-80 PMID: 8989888
  4. The distribution of genes in the human genome.
    Gene. 1991 Apr;100:181-7 PMID: 2055469
  5. Physical mapping of unique nucleotide sequences on identified rice chromosomes.
    Plant Mol Biol. 1998 Dec;38(6):1043-52 PMID: 9869410
  6. The distribution of genes in the genomes of Gramineae.
    Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):6857-61 PMID: 9192656
  7. Rapid isolation of high molecular weight plant DNA.
    Nucleic Acids Res. 1980 Oct 10;8(19):4321-5 PMID: 7433111
  8. A functional S locus anther gene is not required for the self-incompatibility response in Brassica oleracea.
    Plant Cell. 1997 Nov;9(11):2065-76 PMID: 9401128
  9. Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones.
    DNA Res. 1997 Jun 30;4(3):215-30 PMID: 9330910
  10. Expression of a gene for a protein similar to HIV-1 Tat binding protein 1 (TBP1) in floral organs of Brassica rapa.
    Plant Cell Physiol. 1997 Aug;38(8):966-9 PMID: 9327593
  11. The J-domain family and the recruitment of chaperone power.
    Trends Biochem Sci. 1998 Jun;23(6):222-7 PMID: 9644977
  12. The self-incompatibility phenotype in brassica is altered by the transformation of a mutant S locus receptor kinase
    Plant Cell. 1998 Feb;10(2):209-18 PMID: 9490744
  13. Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea.
    Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8816-20 PMID: 1681543
  14. Physical linkage of the SLG and SRK genes at the self-incompatibility locus of Brassica oleracea.
    Mol Gen Genet. 1993 Jan;236(2-3):369-73 PMID: 8437582
  15. High-resolution physical mapping in Arabidopsis thaliana and tomato by fluorescence in situ hybridization to extended DNA fibres.
    Plant J. 1996 Mar;9(3):421-30 PMID: 8919917
  16. Cloning the cDNA encoding the AmbtV allergen from giant ragweed (Ambrosia trifida) pollen.
    Gene. 1991 May 30;101(2):231-8 PMID: 1711499
  17. Distinctive sequence features in protein coding genic non-coding, and intergenic human DNA.
    J Mol Biol. 1995 Oct 13;253(1):51-60 PMID: 7473716
  18. The self-incompatibility (S) haplotypes of Brassica contain highly divergent and rearranged sequences of ancient origin.
    Plant Cell. 1997 Feb;9(2):237-47 PMID: 9061954
  19. Molecular cloning of members of the S-multigene family in self-incompatible Brassica campestris L.
    Plant Cell Physiol. 1995 Oct;36(7):1273-80 PMID: 8564299
  20. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  21. Psl: a novel Spm-like transposable element from Petunia hybrida.
    Plant J. 1998 Apr;14(1):43-54 PMID: 9681025
  22. Two new S-phase-specific genes from Saccharomyces cerevisiae.
    Yeast. 1997 Sep 15;13(11):1029-42 PMID: 9290207
  23. Distribution of genes in the genome of Arabidopsis thaliana and its implications for the genome organization of plants.
    Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10044-9 PMID: 9707597
  24. Prediction of complete gene structures in human genomic DNA.
    J Mol Biol. 1997 Apr 25;268(1):78-94 PMID: 9149143
  25. Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana.
    Nature. 1998 Jan 29;391(6666):485-8 PMID: 9461215
  26. Distinct cis-acting elements direct pistil-specific and pollen-specific activity of the Brassica S locus glycoprotein gene promoter.
    Plant Cell. 1993 Aug;5(8):855-63 PMID: 8400868
  27. A high degree of homology exists between the protein encoded by SLG and the S receptor domain encoded by SRK in self-incompatible Brassica campestris L.
    Plant Cell Physiol. 1994 Dec;35(8):1221-9 PMID: 7697295
  28. An anther-specific gene encoded by an S locus haplotype of Brassica produces complementary and differentially regulated transcripts.
    Plant Cell. 1995 Aug;7(8):1283-94 PMID: 7549484
  29. Direct cloning of the Brassica S locus by using a P1-derived artificial chromosome (PAC) vector.
    Gene. 1997 Oct 15;199(1-2):133-7 PMID: 9358048
  30. Comparative mapping of the Brassica S locus region and its homeolog in Arabidopsis. Implications for the evolution of mating systems in the Brassicaceae.
    Plant Cell. 1998 May;10(5):801-12 PMID: 9596638
  31. Characterization of the S locus genes, SLG and SRK, of the Brassica S3 haplotype: identification of a membrane-localized protein encoded by the S locus receptor kinase gene.
    Plant J. 1995 Mar;7(3):429-40 PMID: 7757115
  32. Cloning of PCP1, a member of a family of pollen coat protein (PCP) genes from Brassica oleracea encoding novel cysteine-rich proteins involved in pollen-stigma interactions.
    Plant J. 1996 Aug;10(2):303-13 PMID: 8771786
  33. An interspecific backcross of Lycopersicon esculentum x L. hirsutum: linkage analysis and a QTL study of sexual compatibility factors and floral traits.
    Genetics. 1997 Oct;147(2):861-77 PMID: 9335620
  34. SRK, the stigma-specific S locus receptor kinase of Brassica, is targeted to the plasma membrane in transgenic tobacco.
    Plant Cell. 1996 Mar;8(3):429-45 PMID: 8721749
  35. An S receptor kinase gene in self-compatible Brassica napus has a 1-bp deletion.
    Plant Cell. 1993 May;5(5):531-9 PMID: 8518554
  36. Alignment and sensitive detection of DNA by a moving interface.
    Science. 1994 Sep 30;265(5181):2096-8 PMID: 7522347
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/153.1.391
Email
gsuzuki@bios.tohoku.ac.jp
Published
1999-09-00
Pages
391-400
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460755
Subset
IM
Databases
GENBANK
AB012866, AB012867, AB022069, AB022070, AB022071, AB022072, AB022073, AB022074, AB022075, AB022076, AB022077, AB022078, AB022079, AB022080, AB022081, AB022082
External Links
PubMed source DOI: 10.1093/genetics/153.1.391 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2