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PMID: 9358048 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Direct cloning of the Brassica S locus by using a P1-derived artificial chromosome (PAC) vector.

Gene ·Vol. 199 ·No. 1-2 ·1997-10-15 ·Pages 133-7

Suzuki G, Watanabe M, Toriyama K, Isogai A, Hinata K

Abstract

Self-incompatibility of Brassica is regulated by the S locus, which contains several genes including SLG and SRK. We found that both SLG and SRK genes were located at an approx. 80-kb MluI fragment in an S9 haplotype of B. campestris. Therefore, we cloned this MluI fragment into a BssHII site of the P1-derived artificial chromosome (PAC) vector. The utility of the direct cloning method is discussed in this study.

MeSH Terms

Bacteria/genetics Bacterial Proteins Brassica/genetics Cloning, Molecular/methods Deoxyribonucleases, Type II Site-Specific Genes, Plant/genetics Genetic Vectors/genetics Glycoproteins/genetics Plant Proteins/genetics Plasmids/genetics Protein Kinases/genetics

Chemicals

Bacterial Proteins Glycoproteins Plant Proteins self-incompatibility locus specific glycoprotein, Brassica Protein Kinases S-receptor kinase endodeoxyribonuclease MluI Deoxyribonucleases, Type II Site-Specific

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Suzuki G

Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, Sendai, Japan.

Watanabe M

Toriyama K

Isogai A

Hinata K

Article Info

Journal

Gene

Abbr.

Gene

ISSN

0378-1119

DOI

10.1016/s0378-1119(97)00358-2

Published

1997-10-15

Pages

133-7

Language

English

Region

Netherlands

NLM ID

7706761

Subset

External Links

PubMed source DOI: 10.1016/s0378-1119(97)00358-2 View journal details

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Direct cloning of the Brassica S locus by using a P1-derived artificial chromosome (PAC) vector.

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