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PMID: 10411133 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The human Prp8 protein is a component of both U2- and U12-dependent spliceosomes.

RNA (New York, N.Y.) ·Vol. 5 ·No. 7 ·1999-07-00 ·Pages 893-908

Luo HR, Moreau GA, Levin N, Moore MJ

Abstract

This study reports the cloning, sequencing, and development of antisera against the human U5 snRNP 220-kDa protein or hPrp8p. Prp8p is the most highly conserved large nuclear protein known to date, but it is not related to any other protein. Southern, Northern, and expressed sequence tag analyses indicate that hPrp8p is encoded by a single gene. Prp8p is a core component of U5 snRNP and the U4/U6.U5 tri-snRNP, and antibodies raised against it immunoprecipitate both the major, U2-dependent and minor, U12-dependent spliceosomes. These spliceosomes, which excise different classes of introns, contain distinct sets of snRNAs overlapping only with U5 snRNA. Other than the core Sm proteins, hPrp8p is the first splicing factor shown to be common to both spliceosomes.

MeSH Terms
Amino Acid Sequence Animals Blotting, Northern Blotting, Southern Blotting, Western Carrier Proteins/genetics,immunology,metabolism Chromosome Mapping Chromosomes, Human, Pair 17 DNA, Complementary Humans Immune Sera Molecular Sequence Data Precipitin Tests RNA, Messenger/genetics RNA-Binding Proteins Ribonucleoprotein, U2 Small Nuclear/metabolism Ribonucleoproteins, Small Nuclear/metabolism Sequence Homology, Amino Acid Spliceosomes/metabolism
Chemicals
Carrier Proteins DNA, Complementary Immune Sera PRPF8 protein, human RNA, Messenger RNA-Binding Proteins Ribonucleoprotein, U2 Small Nuclear Ribonucleoproteins, Small Nuclear U11-U12 small nuclear ribonucleoprotein
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Luo H R
Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454-9110, USA.
Moreau G A
Levin N
Moore M J
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1017/s1355838299990520
Published
1999-07-00
Pages
893-908
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1369814
Subset
IM
External Links
PubMed source DOI: 10.1017/s1355838299990520 PMC Full Text View journal details
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