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PMID: 10369682 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ptx1 regulates SF-1 activity by an interaction that mimics the role of the ligand-binding domain.

The EMBO journal ·Vol. 18 ·No. 12 ·1999-06-15 ·Pages 3431-41

Tremblay JJ, Marcil A, Gauthier Y, Drouin J

Abstract

Ptx1 (Pitx1) is a bicoid-related homeobox transcription factor expressed from the onset of pituitary development. It was shown to cooperate with cell-restricted factors, such as Pit1, NeuroD1/PanI and steroidogenic factor 1 (SF-1), to establish a combinatorial code conferring lineage- and promoter-specific gene transcription in the pituitary. Transcriptional synergism between Ptx1 and SF-1 on two SF-1 target genes, pituitary luteinizing hormone beta and Müllerian-inhibiting substance (MIS), requires SF-1 binding to DNA and appears to result from direct physical interaction between these two proteins. The interaction between the C-terminus of Ptx1 and the N-terminal half of SF-1 results in transcriptional enhancement that equals the activity of a constitutively active SF-1 mutant and that may mimic the effect of a still unidentified SF-1 ligand. Thus, the unmasking of SF-1 activity by Ptx1 may represent a developmental mechanism to alleviate the need for SF-1 ligand in transcription and, possibly, at critical times during organogenesis.

MeSH Terms
Animals Anti-Mullerian Hormone Binding Sites Cell Line Chlorocebus aethiops DNA/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Fushi Tarazu Transcription Factors Glycoproteins Growth Inhibitors/genetics Homeodomain Proteins/genetics,metabolism L Cells Ligands Luteinizing Hormone/genetics Mice Models, Genetic Paired Box Transcription Factors Promoter Regions, Genetic/genetics Protein Binding Receptors, Cytoplasmic and Nuclear Response Elements/genetics Sequence Deletion Steroidogenic Factor 1 Testicular Hormones/genetics Transcription Factors/genetics,metabolism Transcriptional Activation Transfection
Chemicals
DNA-Binding Proteins Fushi Tarazu Transcription Factors Glycoproteins Growth Inhibitors Homeodomain Proteins Ligands Paired Box Transcription Factors Receptors, Cytoplasmic and Nuclear Steroidogenic Factor 1 Testicular Hormones Transcription Factors homeobox protein PITX1 steroidogenic factor 1, mouse Anti-Mullerian Hormone Luteinizing Hormone DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tremblay J J
Laboratoire de Génétique Moléculaire, Institut de Recherches Cliniques de Montréal, 110 des Pins Ouest, Montréal, Québec, Canada H2W 1R7.
Marcil A
Gauthier Y
Drouin J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/18.12.3431
Published
1999-06-15
Pages
3431-41
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1171422
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/18.12.3431 PMC Full Text View journal details
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