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PMID: 10369677 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Catabolite control of Escherichia coli regulatory protein BglG activity by antagonistically acting phosphorylations.

The EMBO journal ·Vol. 18 ·No. 12 ·1999-06-15 ·Pages 3370-9

Görke B, Rak B

Abstract

In bacteria various sugars are taken up and concomitantly phosphorylated by sugar-specific enzymes II (EII) of the phosphoenolpyruvate:sugar phosphotransferase system (PTS). The phosphoryl groups are donated by the phosphocarrier protein HPr. BglG, the positively acting regulatory protein of the Escherichia coli bgl (beta-glucoside utilization) operon, is known to be negatively regulated by reversible phosphorylation catalyzed by the membrane spanning beta-glucoside-specific EIIBgl. Here we present evidence that in addition BglG must be phosphorylated by HPr at a distinct site to gain activity. Our data suggest that this second, shortcut route of phosphorylation is used to monitor the state of the various PTS sugar availabilities in order to hierarchically tune expression of the bgl operon in a physiologically meaningful way. Thus, the PTS may represent a highly integrated signal transduction network in carbon catabolite control.

MeSH Terms
Bacterial Proteins/antagonists & inhibitors,chemistry,genetics,metabolism Benzyl Alcohols/metabolism Biological Transport Carbohydrate Metabolism Carbon/metabolism Carrier Proteins/genetics,metabolism Down-Regulation Escherichia coli/enzymology,genetics,growth & development,metabolism Escherichia coli Proteins Gene Expression Regulation, Bacterial Genetic Complementation Test Glucosides Intracellular Signaling Peptides and Proteins Isopropyl Thiogalactoside Models, Biological Mutation Operon/genetics Phosphates/metabolism Phosphoenolpyruvate Sugar Phosphotransferase System/genetics,metabolism Phosphorylation Protein Kinases RNA-Binding Proteins/antagonists & inhibitors,chemistry,genetics,metabolism Signal Transduction Terminator Regions, Genetic/genetics
Chemicals
Bacterial Proteins Benzyl Alcohols Carrier Proteins Escherichia coli Proteins Glucosides Intracellular Signaling Peptides and Proteins Phosphates RNA-Binding Proteins antiterminator proteins, Bacteria fructose-induced HPr-like protein, bacteria Isopropyl Thiogalactoside salicin Carbon Protein Kinases fruB protein, E coli Phosphoenolpyruvate Sugar Phosphotransferase System phosphocarrier protein HPr
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Görke B
Institut für Biologie III, Universität, Schänzlestrasse 1, D-79104 Freiburg, Germany.
Rak B
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/18.12.3370
Published
1999-06-15
Pages
3370-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1171417
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/18.12.3370 PMC Full Text View journal details
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