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PMID: 10369663 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The mechanism mediating regenerative intercellular Ca2+ waves in the blowfly salivary gland.

The EMBO journal ·Vol. 18 ·No. 12 ·1999-06-15 ·Pages 3222-31

Zimmermann B, Walz B

Abstract

Intercellular Ca2+ signaling in intact salivary glands of the blowfly Calliphora erythrocephala was studied by fluorimetric digital imaging combined with microinjection of putative messenger molecules. Iontophoretic injection of D-myo-inositol 1,4, 5-trisphosphate (InsP3) into salivary gland cells evoked regenerative intercellular Ca2+ waves that spread through the impaled cell and several rows of surrounding cells. Ca2+ increases induced by microinjection of Ca2+ ions were confined to the injected cells and their nearest neighbors. Depletion of intracellular Ca2+ stores by thapsigargin pre-treatment did not alter the time course of the Ca2+ increase caused by Ca2+ injection. However, activation of Ca2+ release became clearly evident when Ca2+ was injected in the presence of serotonin (5-HT). Under these conditions, injection of Ca2+ triggered intercellular Ca2+ waves that consecutively passed through >10 cells. The phospholipase C inhibitor U73122 blocked 5-HT-induced Ca2+ increases but did not affect InsP3-dependent Ca2+ spiking and intercellular Ca2+ wave propagation. The results demonstrate that propagation of agonist-evoked Ca2+ waves in the blowfly salivary gland requires supra-basal [InsP3] but does not depend on feedback activation of phospholipase C. We conclude that the intra- and intercellular transmission of these Ca2+ waves is mediated by diffusion of Ca2+ and Ca2+-induced Ca2+ release via the InsP3 receptor channel.

MeSH Terms
Animals Calcium/administration & dosage,metabolism,pharmacology Calcium Channels/physiology Calcium Signaling/drug effects Diffusion Diptera/metabolism Enzyme Activation Estrenes/pharmacology Feedback In Vitro Techniques Inositol 1,4,5-Trisphosphate/administration & dosage,pharmacology Inositol 1,4,5-Trisphosphate Receptors Microinjections Pyrrolidinones/pharmacology Receptors, Cytoplasmic and Nuclear/physiology Salivary Glands/cytology,drug effects,enzymology,metabolism Serotonin/pharmacology Serotonin Antagonists/pharmacology Thapsigargin/pharmacology Time Factors Type C Phospholipases/antagonists & inhibitors,metabolism
Chemicals
Calcium Channels Estrenes Inositol 1,4,5-Trisphosphate Receptors Pyrrolidinones Receptors, Cytoplasmic and Nuclear Serotonin Antagonists 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione Serotonin Thapsigargin Inositol 1,4,5-Trisphosphate Type C Phospholipases Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zimmermann B
Institut für Zoophysiologie und Zellbiologie, Universität Potsdam, D-14471 Potsdam, Germany. zimmerm@rz.uni-potsdam.de
Walz B
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/18.12.3222
Email
zimmerm@rz.uni-potsdam.de
Published
1999-06-15
Pages
3222-31
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1171403
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/18.12.3222 PMC Full Text View journal details
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