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PMID: 10359775 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 12 ·1999-06-08 ·Pages 6700-4

Peleg G, Lewis A, Linial M, Loew LM

Abstract

Membrane potential around single molecules has been measured by using the nonlinear optical phenomenon of second harmonic generation. This advance results from the interaction between a highly dipolar molecule with a selectively directed highly polarizable 1-nm gold particle. With this approach, a second harmonic signal, which is enhanced by the nanoparticle, is detected from a volume of nanometric dimensions. This present work clearly shows that functional cellular imaging around single molecules is possible by selectively directing an antibody with a 1-nm gold label to a specific membrane protein. The results of this work open the way for three-dimensional, high resolution functional imaging of membrane electrophysiology in cells and cellular networks.

MeSH Terms

Animals Antigens, Surface/chemistry Cell Line Cell Membrane/chemistry,physiology Coloring Agents Membrane Potentials Microscopy/methods Neurons/chemistry,physiology

Chemicals

Antigens, Surface Coloring Agents

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Peleg G

Division of Applied Physics, Center for Neural Computation, Department of Ophthalmology, Hadassah Laser Center, Hebrew University of Jerusalem, Jerusalem 91904, Israel.

Lewis A

Linial M

Loew L M

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.96.12.6700

Published

1999-06-08

Pages

6700-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC21978

Subset

External Links

PubMed source DOI: 10.1073/pnas.96.12.6700 PMC Full Text View journal details

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Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites.

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