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PMID: 10359608 Published · ppublish English Journal Article

Vesicle-associated membrane protein 4 is implicated in trans-Golgi network vesicle trafficking.

Molecular biology of the cell ·Vol. 10 ·No. 6 ·1999-06-00 ·Pages 1957-72

Steegmaier M, Klumperman J, Foletti DL, Yoo JS, Scheller RH

Abstract

The trans-Golgi network (TGN) plays a pivotal role in directing proteins in the secretory pathway to the appropriate cellular destination. VAMP4, a recently discovered member of the vesicle-associated membrane protein (VAMP) family of trafficking proteins, has been suggested to play a role in mediating TGN trafficking. To better understand the function of VAMP4, we examined its precise subcellular distribution. Indirect immunofluorescence and electron microscopy revealed that the majority of VAMP4 localized to tubular and vesicular membranes of the TGN, which were in part coated with clathrin. In these compartments, VAMP4 was found to colocalize with the putative TGN-trafficking protein syntaxin 6. Additional labeling was also present on clathrin-coated and noncoated vesicles, on endosomes and the medial and trans side of the Golgi complex, as well as on immature secretory granules in PC12 cells. Immunoprecipitation of VAMP4 from rat brain detergent extracts revealed that VAMP4 exists in a complex containing syntaxin 6. Converging lines of evidence implicate a role for VAMP4 in TGN-to-endosome transport.

MeSH Terms
Animals Biological Transport Brain/cytology Brefeldin A/pharmacology Clathrin/metabolism Coated Vesicles/drug effects,metabolism Detergents/chemistry Fluorescent Antibody Technique, Indirect Golgi Apparatus/drug effects,metabolism,ultrastructure Intracellular Membranes/metabolism Membrane Proteins/chemistry,drug effects,metabolism Microscopy, Electron Proteins/isolation & purification Qa-SNARE Proteins Rats Subcellular Fractions
Chemicals
Clathrin Detergents Membrane Proteins Proteins Qa-SNARE Proteins Brefeldin A
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Steegmaier M
Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305-5345, USA.
Klumperman J
Foletti D L
Yoo J S
Scheller R H
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.10.6.1957
Published
1999-06-00
Pages
1957-72
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25394
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.10.6.1957 PMC Full Text View journal details
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