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PMID: 10357820 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Repression by Ikaros and Aiolos is mediated through histone deacetylase complexes.

The EMBO journal ·Vol. 18 ·No. 11 ·1999-06-01 ·Pages 3090-100

Koipally J, Renold A, Kim J, Georgopoulos K

Abstract

Here we show that the lymphoid lineage-determining factors Ikaros and Aiolos can function as strong transcriptional repressors. This function is mediated through two repression domains and is dependent upon the promoter context and cell type. Repression by Ikaros proteins correlates with hypo-acetylation of core histones at promoter sites and is relieved by histone deacetylase inhibitors. Consistent with these findings, Ikaros and its repression domains can interact in vivo and in vitro with the mSin3 family of co-repressors which bind to histone deacetylases. Based on these and our recent findings of associations between Ikaros and Mi-2-HDAC, we propose that Ikaros family members modulate gene expression during lymphocyte development by recruiting distinct histone deacetylase complexes to specific promoters.

MeSH Terms
Acetylation Amino Acid Sequence Animals Binding Sites Cell Line Chromatin/genetics,metabolism DNA-Binding Proteins Histone Deacetylase Inhibitors Histone Deacetylases/metabolism Histones/metabolism Humans Ikaros Transcription Factor Lymphocytes/cytology,metabolism Mice Molecular Sequence Data Promoter Regions, Genetic/genetics Protein Binding Protein Isoforms/chemistry,genetics,metabolism RNA, Messenger/analysis,genetics,metabolism Repressor Proteins/chemistry,genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Deletion Trans-Activators/chemistry,genetics,metabolism Transcription Factors/chemistry,genetics,metabolism Transcription, Genetic/genetics Yeasts/genetics,metabolism
Chemicals
Chromatin DNA-Binding Proteins Histone Deacetylase Inhibitors Histones IKZF1 protein, human IKZF3 protein, human Ikzf3 protein, mouse Protein Isoforms RNA, Messenger Repressor Proteins SIN3 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors Zfpn1a1 protein, mouse Ikaros Transcription Factor Histone Deacetylases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Koipally J
Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.
Renold A
Kim J
Georgopoulos K
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/18.11.3090
Published
1999-06-01
Pages
3090-100
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1171390
Subset
IM
Grants
NIAID NIH HHS · R01 AI033062 · United States
NIAID NIH HHS · R01 AI33062 · United States
External Links
PubMed source DOI: 10.1093/emboj/18.11.3090 PMC Full Text View journal details
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