Home LiteratureArticle Details
PMID: 10220408 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Pathways of As(III) detoxification in Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 9 ·1999-04-27 ·Pages 5001-6

Ghosh M, Shen J, Rosen BP

Abstract

Saccharomyces cerevisiae has two independent transport systems for the removal of arsenite from the cytosol. Acr3p is a plasma membrane transporter that confers resistance to arsenite, presumably by arsenite extrusion from the cells. Ycf1p, a member of the ABC transporter superfamily, catalyzes the ATP-driven uptake of As(III) into the vacuole, also producing resistance to arsenite. Vacuolar accumulation requires a reductant such as glutathione, suggesting that the substrate is the glutathione conjugate, As(GS)3. Disruption of either the ACR3 or YCF1 gene results in sensitivity to arsenite and disruption of both genes produces additive hypersensitivity. Thus, Acr3p and Ycf1p represent separate pathways for the detoxification of arsenite in yeast.

MeSH Terms
ATP-Binding Cassette Transporters/metabolism Arsenites/metabolism Biodegradation, Environmental Biological Transport Fungal Proteins/metabolism Membrane Proteins/metabolism Membrane Transport Proteins Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Signal Transduction
Chemicals
ACR3 protein, S cerevisiae ATP-Binding Cassette Transporters Arsenites Fungal Proteins Membrane Proteins Membrane Transport Proteins Saccharomyces cerevisiae Proteins YCF1 protein, S cerevisiae arsenite
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ghosh M
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Shen J
Rosen B P
References (40)
40 references, click to expand
  1. A yeast metal resistance protein similar to human cystic fibrosis transmembrane conductance regulator (CFTR) and multidrug resistance-associated protein.
    J Biol Chem. 1994 Sep 9;269(36):22853-7 PMID: 7521334
  2. Arsenate reductase of Staphylococcus aureus plasmid pI258.
    Biochemistry. 1994 Jun 14;33(23):7294-9 PMID: 8003493
  3. The MRP gene encodes an ATP-dependent export pump for leukotriene C4 and structurally related conjugates.
    J Biol Chem. 1994 Nov 11;269(45):27807-10 PMID: 7961706
  4. Cadmium tolerance mediated by the yeast AP-1 protein requires the presence of an ATP-binding cassette transporter-encoding gene, YCF1.
    J Biol Chem. 1994 Dec 23;269(51):32592-7 PMID: 7798263
  5. High level arsenite resistance in Leishmania tarentolae is mediated by an active extrusion system.
    Mol Biochem Parasitol. 1994 Sep;67(1):49-57 PMID: 7838183
  6. New mechanisms of drug resistance in parasitic protozoa.
    Annu Rev Microbiol. 1995;49:427-60 PMID: 8561467
  7. Efflux-mediated resistance to arsenicals in arsenic-resistant and -hypersensitive Chinese hamster cells.
    Toxicol Appl Pharmacol. 1996 Mar;137(1):112-9 PMID: 8607136
  8. The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump.
    J Biol Chem. 1996 Mar 15;271(11):6509-17 PMID: 8626454
  9. The human multidrug resistance-associated protein functionally complements the yeast cadmium resistance factor 1.
    Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6743-8 PMID: 8692889
  10. An ATP-dependent As(III)-glutathione transport system in membrane vesicles of Leishmania tarentolae.
    Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):2192-7 PMID: 8700907
  11. A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae: YCF1-catalyzed transport of bis(glutathionato)cadmium.
    Proc Natl Acad Sci U S A. 1997 Jan 7;94(1):42-7 PMID: 8990158
  12. PMR1, a Ca2+-ATPase in yeast Golgi, has properties distinct from sarco/endoplasmic reticulum and plasma membrane calcium pumps.
    J Biol Chem. 1997 Apr 11;272(15):9895-901 PMID: 9092527
  13. Isolation of three contiguous genes, ACR1, ACR2 and ACR3, involved in resistance to arsenic compounds in the yeast Saccharomyces cerevisiae.
    Yeast. 1997 Jul;13(9):819-28 PMID: 9234670
  14. Characterization of phosphine complexes of technetium(III) as transport substrates of the multidrug resistance P-glycoprotein and functional markers of P-glycoprotein at the blood-brain barrier.
    Biochemistry. 1997 Nov 18;36(46):14218-27 PMID: 9369495
  15. The Saccharomyces cerevisiae ACR3 gene encodes a putative membrane protein involved in arsenite transport.
    J Biol Chem. 1997 Nov 28;272(48):30061-6 PMID: 9374482
  16. The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus.
    Nature. 1997 Nov 27;390(6658):364-70 PMID: 9389475
  17. Complete genome structure of the unicellular cyanobacterium Synechocystis sp. PCC6803.
    Plant Cell Physiol. 1997 Nov;38(11):1171-6 PMID: 9435137
  18. Metalloid resistance mechanisms in prokaryotes.
    J Biochem. 1998 Jan;123(1):16-23 PMID: 9504403
  19. Acute promyelocytic leukemia: cellular and molecular basis of differentiation and apoptosis.
    Pharmacol Ther. 1997 Oct-Dec;76(1-3):141-9 PMID: 9535176
  20. The ars operon in the skin element of Bacillus subtilis confers resistance to arsenate and arsenite.
    J Bacteriol. 1998 Apr;180(7):1655-61 PMID: 9537360
  21. An ABC-transporter of Arabidopsis thaliana has both glutathione-conjugate and chlorophyll catabolite transport activity.
    Plant J. 1998 Mar;13(6):773-80 PMID: 9681016
  22. Complete remission after treatment of acute promyelocytic leukemia with arsenic trioxide.
    N Engl J Med. 1998 Nov 5;339(19):1341-8 PMID: 9801394
  23. Saccharomyces cerevisiae ACR2 gene encodes an arsenate reductase.
    FEMS Microbiol Lett. 1998 Nov 1;168(1):127-36 PMID: 9812373
  24. Determination of microgram quantities of protein in the presence of milligram levels of lipid with amido black 10B.
    Anal Biochem. 1985 Oct;150(1):97-104 PMID: 4083487
  25. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  26. Preparation and purification of 74As-labeled arsenate and arsenite for use in biological experiments.
    Anal Biochem. 1977 Apr;78(2):557-60 PMID: 851228
  27. Kinetic mechanism of mitochondrial adenosine triphosphatase. Inhibition by azide and activation by sulphite.
    Biochem J. 1982 Jan 15;202(1):15-23 PMID: 6211171
  28. Energetics of plasmid-mediated arsenate resistance in Escherichia coli.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6119-22 PMID: 6755463
  29. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  30. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  31. Yeast/E. coli shuttle vectors with multiple unique restriction sites.
    Yeast. 1986 Sep;2(3):163-7 PMID: 3333305
  32. Trypanothione is the primary target for arsenical drugs against African trypanosomes.
    Proc Natl Acad Sci U S A. 1989 Apr;86(8):2607-11 PMID: 2704738
  33. Membrane protein sorting: biosynthesis, transport and processing of yeast vacuolar alkaline phosphatase.
    EMBO J. 1989 Aug;8(8):2241-50 PMID: 2676517
  34. Novel biochemical pathways in parasitic protozoa.
    Parasitology. 1989;99 Suppl:S93-112 PMID: 2682488
  35. Characterization of ATP12, a yeast nuclear gene required for the assembly of the mitochondrial F1-ATPase.
    J Biol Chem. 1991 Apr 25;266(12):7517-23 PMID: 1826907
  36. Drug resistance and P-glycoprotein gene amplification in the protozoan parasite Leishmania.
    Res Microbiol. 1991 Jul-Aug;142(6):737-46 PMID: 1961984
  37. Drug resistance in leishmaniasis: its implication in systemic chemotherapy of cutaneous and mucocutaneous disease.
    Am J Trop Med Hyg. 1992 Jul;47(1):117-26 PMID: 1322070
  38. Reduction and binding of arsenate and dimethylarsinate by glutathione: a magnetic resonance study.
    Chem Biol Interact. 1994 Feb;90(2):139-55 PMID: 8156604
  39. Properties of the arsenate reductase of plasmid R773.
    Biochemistry. 1994 Jun 14;33(23):7288-93 PMID: 8003492
  40. Pharmacological characterization of multidrug resistant MRP-transfected human tumor cells.
    Cancer Res. 1994 Nov 15;54(22):5902-10 PMID: 7954421
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.9.5001
Published
1999-04-27
Pages
5001-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC21806
Subset
IM
Grants
NIGMS NIH HHS · R01 GM055425 · United States
NIGMS NIH HHS · R37 GM055425 · United States
NIGMS NIH HHS · GM55425 · United States
External Links
PubMed source DOI: 10.1073/pnas.96.9.5001 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2