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PMID: 10205174 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The C-terminal domain of armadillo binds to hypophosphorylated teashirt to modulate wingless signalling in Drosophila.

The EMBO journal ·Vol. 18 ·No. 8 ·1999-04-15 ·Pages 2208-17

Gallet A, Angelats C, Erkner A, Charroux B, Fasano L, Kerridge S

Abstract

Wnt signalling is a key pathway for tissue patterning during animal development. In Drosophila, the Wnt protein Wingless acts to stabilize Armadillo inside cells where it binds to at least two DNA-binding factors which regulate specific target genes. One Armadillo-binding protein in Drosophila is the zinc finger protein Teashirt. Here we show that Wingless signalling promotes the phosphorylation and the nuclear accumulation of Teashirt. This process requires the binding of Teashirt to the C-terminal end of Armadillo. Finally, we present evidence that the serine/threonine kinase Shaggy is associated with Teashirt in a complex. We discuss these results with respect to current models of Armadillo/beta-catenin action for the transmission of the Wingless/Wnt pathway.

MeSH Terms
Amino Acid Sequence Animals Armadillo Domain Proteins Drosophila/genetics,metabolism Drosophila Proteins Insect Proteins/chemistry,metabolism Molecular Sequence Data Phosphorylation Protein Binding Proto-Oncogene Proteins/metabolism Repressor Proteins Sequence Homology, Amino Acid Signal Transduction Subcellular Fractions/metabolism Trans-Activators Transcription Factors/metabolism Wnt1 Protein
Chemicals
ARM protein, Drosophila Armadillo Domain Proteins Drosophila Proteins Insect Proteins Proto-Oncogene Proteins Repressor Proteins Trans-Activators Transcription Factors Wnt1 Protein wg protein, Drosophila tsh protein, Drosophila
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gallet A
Labororatoire de Génétique et Physiologie du Développement, UMR 9943 CNRS-Université, IBDM CNRS-INSERM-Université de la Méditerranée, Campus de Luminy Case 907, F-13288 Marseille, Cedex 09, France.
Angelats C
Erkner A
Charroux B
Fasano L
Kerridge S
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/18.8.2208
Published
1999-04-15
Pages
2208-17
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1171304
Subset
IM
Grants
NICHD NIH HHS · N01-HD-2-3144 · United States
External Links
PubMed source DOI: 10.1093/emboj/18.8.2208 PMC Full Text View journal details
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