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PMID: 10203476 Published · ppublish English Journal Article

Phenotypic and genomic variation among Staphylococcus epidermidis strains infecting joint prostheses.

Journal of clinical microbiology ·Vol. 37 ·No. 5 ·1999-05-00 ·Pages 1306-12

Galdbart JO, Morvan A, Desplaces N, el Solh N

Abstract

We studied the SmaI and SstII macrorestriction patterns of 54 Staphylococcus epidermidis strains isolated from 14 patients infected following the implantation of joint prostheses. Multiple strains from pus and infected tissue specimens of each patient were selected on the basis of different colony morphologies and drug resistance patterns. The same criteria were used to select 23 S. epidermidis strains from hand swabs of eight healthy individuals. For 10 of the 14 patients, all the intrapatient strains appeared to be closely or possibly related, whereas related strains were detected in the skin flora of only one of the eight healthy individuals. This observation suggests that, in most cases, the patients were infected by a single S. epidermidis clone which subsequently underwent rearrangements that yielded derivatives with divergent phenotypes and, occasionally, divergent macrorestriction patterns. The four patients whose specimens contained unrelated S. epidermidis strains were probably infected with several polyclonal strains.

MeSH Terms
Arthroplasty, Replacement/adverse effects Drug Resistance, Microbial Genotype Humans Phenotype Postoperative Complications/microbiology Skin/microbiology Staphylococcus epidermidis/classification,drug effects,genetics
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Galdbart J O
Unité des Staphylocoques, National Reference Center for Staphylococci, Institut Pasteur, 72724 Paris Cedex 15, France.
Morvan A
Desplaces N
el Solh N
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.37.5.1306-1312.1999
Published
1999-05-00
Pages
1306-12
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC84759
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.37.5.1306-1312.1999 PMC Full Text View journal details
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