Home LiteratureArticle Details
PMID: 101511 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Phospholipid composition of Rickettsia prowazeki grown in chicken embryo yolk sacs.

Journal of bacteriology ·Vol. 136 ·No. 1 ·1978-10-00 ·Pages 175-8

Winkler HH, Miller ET

Abstract

The phospholipid composition and phospholipid fatty acid composition of purified Rickettsia prowazeki were determined. The lipid phosphorous content was 6.8 +/- 1.3 microgram/mg of total rickettsial protein. The major phospholipid was phosphatidylethanolamine (60 to 70%); phosphatidylglycerol constituted 20%, and phosphatidylcholine constituted 15%. Small amounts of phosphatidylserine and cardiolipin were detected. The principal fatty acids were 18:1, 16:1, and 16:0. The fatty acid composition of the phosphatidylcholine in the rickettsial extracts was very different than that of the other rickettsial phosphatides and very similar to that of normal yolk sac phosphatidylcholine. The specific of the phosphatidylcholine of rickettsiae grown in the presence of 32P was markedly lower than that of phosphatidylethanolamine and phosphatidylglycerol. It is suggested that the phosphatidylcholine in the rickettsial extract is yolk sac derived and either tightly absorbed or exchanged into the rickettsial membrane.

MeSH Terms
Cardiolipins/analysis Fatty Acids/analysis Phosphatidylcholines/analysis Phosphatidylethanolamines/analysis Phosphatidylglycerols/analysis Phosphatidylserines/analysis Phospholipids/analysis Rickettsia prowazekii/analysis,growth & development Yolk Sac/microbiology
Chemicals
Cardiolipins Fatty Acids Phosphatidylcholines Phosphatidylethanolamines Phosphatidylglycerols Phosphatidylserines Phospholipids
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Winkler H H
Miller E T
References (21)
21 references, click to expand
  1. Surface distribution of the fatty acid side chains of phosphatidylethanolamine in mixed phospholipid vesicles.
    Biochim Biophys Acta. 1975 Dec 1;413(2):157-62 PMID: 1238121
  2. Nucleic acids of Rickettsiae.
    Nature. 1952 Nov 15;170(4333):846-7 PMID: 13013228
  3. The epidemiology of Rocky Mountain spotted fever. I. The characterization of strain virulence of Rickettsia rickettsii.
    Am J Hyg. 1953 Sep;58(2):248-68 PMID: 13080263
  4. A rapid method of total lipid extraction and purification.
    Can J Biochem Physiol. 1959 Aug;37(8):911-7 PMID: 13671378
  5. Rickettsial permeability. An ADP-ATP transport system.
    J Biol Chem. 1976 Jan 25;251(2):389-96 PMID: 1389
  6. Cell-wall constituents of rickettsiae and psittacosis-lymphogranuloma organisms.
    J Gen Microbiol. 1963 Mar;30:469-80 PMID: 13942448
  7. THE LOCATION OF THE MUCOPEPTIDE IN SECTIONS OF THE CELL WALL OF ESCHERICHIA COLI AND OTHER GRAM-NEGATIVE BACTERIA.
    Can J Microbiol. 1965 Jun;11:547-60 PMID: 14346132
  8. Metabolic studies of rickettsiae. I. The effects of antimicrobial substances and enzyme inhibitors on the oxidation of glutamate by purified rickettsiae.
    J Immunol. 1951 Aug;67(2):123-36 PMID: 14861424
  9. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  10. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae.
    J Bacteriol. 1950 Apr;59(4):509-22 PMID: 15436424
  11. EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI.
    J Bacteriol. 1962 Dec;84(6):1260-7 PMID: 16561982
  12. Fine structure of Rickettsia canada in tissues of Dermacentor andersoni Stiles.
    J Bacteriol. 1971 Mar;105(3):1149-59 PMID: 4100833
  13. Distribution of lipids in the wall and cytoplasmic membrane subfractions of the cell envelope of Escherichia coli.
    J Bacteriol. 1972 Feb;109(2):686-90 PMID: 4550815
  14. DNA base composition of rickettsiae.
    Science. 1973 Apr 27;180(4084):415-7 PMID: 4633692
  15. Electron microscopy studies of the limiting layers of the rickettsia Coxiella burneti.
    J Bacteriol. 1975 Apr;122(1):316-24 PMID: 47324
  16. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism.
    J Bacteriol. 1968 Mar;95(3):833-43 PMID: 4868364
  17. Details of the ultrastructure of Rickettsia prowazekii grown in the chick yolk sac.
    J Bacteriol. 1967 Jul;94(1):260-2 PMID: 4961414
  18. The cell wall of Rickettsia mooseri. I. Morphology and chemical composition.
    J Bacteriol. 1967 Mar;93(3):1113-8 PMID: 6025416
  19. Characterization of the Madrid E strain of Rickettsia prowazekii purified by renografin density gradient centrifugation.
    Infect Immun. 1977 Jan;15(1):280-6 PMID: 64456
  20. Separation of viable Rickettsia typhi from yolk sac and L cell host components by renografin density gradient centrifugation.
    Appl Microbiol. 1975 Sep;30(3):456-63 PMID: 810089
  21. Proteins of typhus and spotted fever group rickettsiae.
    Infect Immun. 1976 Jul;14(1):155-62 PMID: 820639
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.136.1.175-178.1978
Published
1978-10-00
Pages
175-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218647
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.136.1.175-178.1978 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2