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PMID: 10085009 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The rgg gene of Streptococcus pyogenes NZ131 positively influences extracellular SPE B production.

Infection and immunity ·Vol. 67 ·No. 4 ·1999-04-00 ·Pages 1715-22

Chaussee MS, Ajdic D, Ferretti JJ

Abstract

Streptococcus pyogenes produces several extracellular proteins, including streptococcal erythrogenic toxin B (SPE B), also known as streptococcal pyrogenic exotoxin B and streptococcal proteinase. Several reports suggest that SPE B contributes to the virulence associated with S. pyogenes; however, little is known about its regulation. Nucleotide sequence data revealed the presence, upstream of the speB gene, of a gene, designated rgg, that was predicted to encode a polypeptide similar to previously described positive regulatory factors. The putative Rgg polypeptide of S. pyogenes NZ131 consisted of 280 amino acids and had a predicted molecular weight of 33,246. To assess the potential role of Rgg in the production of SPE B, the rgg gene was insertionally inactivated in S. pyogenes NZ131, which resulted in markedly decreased SPE B production, as determined both by immunoblotting and caseinolytic activity on agar plates. However, the production of other extracellular products, including streptolysin O, streptokinase, and DNase, was not affected. Complementation of the rgg mutant with an intact rgg gene copy in S. pyogenes NZ131 could restore SPE B production and confirmed that the rgg gene product is involved in the production of SPE B.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/biosynthesis,genetics Base Sequence DNA, Bacterial DNA-Binding Proteins Deoxyribonucleases/metabolism Exotoxins/biosynthesis Gene Expression Regulation, Bacterial Genetic Complementation Test Hemolysis Membrane Proteins Molecular Sequence Data Mutagenesis Polymerase Chain Reaction/methods Sequence Analysis, DNA Sequence Homology, Amino Acid Streptococcus pyogenes/genetics,metabolism Streptokinase/metabolism Trans-Activators
Chemicals
Bacterial Proteins DNA, Bacterial DNA-Binding Proteins Exotoxins Membrane Proteins SpeA protein, Streptococcus pyogenes Trans-Activators erythrogenic toxin rgg protein, Streptococcus Deoxyribonucleases Streptokinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chaussee M S
Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA. mchaussee@nih.gov
Ajdic D
Ferretti J J
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.67.4.1715-1722.1999
Email
mchaussee@nih.gov
Published
1999-04-00
Pages
1715-22
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC96519
Subset
IM
Grants
NIAID NIH HHS · AI38406 · United States
Databases
GENBANK
AF091252
External Links
PubMed source DOI: 10.1128/IAI.67.4.1715-1722.1999 PMC Full Text View journal details
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