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PMID: 10074071 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Uracil-induced down-regulation of the yeast uracil permease.

Journal of bacteriology ·Vol. 181 ·No. 6 ·1999-03-00 ·Pages 1793-800

Séron K, Blondel MO, Haguenauer-Tsapis R, Volland C

Abstract

In Saccharomyces cerevisiae the FUR4-encoded uracil permease catalyzes the first step of the pyrimidine salvage pathway. The availability of uracil has a negative regulatory effect upon its own transport. Uracil causes a decrease in the level of uracil permease, partly by decreasing the FUR4 mRNA level in a promoter-independent fashion, probably by increasing its instability. Uracil entry also triggers more rapid degradation of the existing permease by promoting high efficiency of ubiquitination of the permease that signals its internalization. A direct binding of intracellular uracil to the permease is possibly involved in this feedback regulation, as the behavior of the permease is similar in mutant cells unable to convert intracellular uracil into UMP. We used cells impaired in the ubiquitination step to show that the addition of uracil produces rapid inhibition of uracil transport. This may be the first response prior to the removal of the permease from the plasma membrane. Similar down-regulation of uracil uptake, involving several processes, was observed under adverse conditions mainly corresponding to a decrease in the cellular content of ribosomes. These results suggest that uracil of exogenous or catabolic origin down-regulates the cognate permease to prevent buildup of excess intracellular uracil-derived nucleotides.

MeSH Terms
Base Sequence DNA Primers/genetics Down-Regulation/drug effects Genes, Fungal Membrane Transport Modulators Membrane Transport Proteins/antagonists & inhibitors,genetics,metabolism Nucleotide Transport Proteins Plasmids/genetics Pyrimidines/pharmacology RNA, Fungal/genetics,metabolism RNA, Messenger/genetics,metabolism Saccharomyces cerevisiae/drug effects,genetics,metabolism Saccharomyces cerevisiae Proteins Up-Regulation Uracil/metabolism,pharmacology
Chemicals
DNA Primers FUR4 protein, S cerevisiae Membrane Transport Modulators Membrane Transport Proteins Nucleotide Transport Proteins Pyrimidines RNA, Fungal RNA, Messenger Saccharomyces cerevisiae Proteins Uracil
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Séron K
Institut Jacques Monod, CNRS/Université Paris 7-Denis Diderot 2, 75251 Paris Cedex 05, France.
Blondel M O
Haguenauer-Tsapis R
Volland C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.6.1793-1800.1999
Published
1999-03-00
Pages
1793-800
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC93577
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.181.6.1793-1800.1999 PMC Full Text View journal details
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