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PMID: 10051598 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interferon-induced human MxA GTPase blocks nuclear import of Thogoto virus nucleocapsids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 5 ·1999-03-02 ·Pages 2082-6

Kochs G, Haller O

Abstract

Interferon-induced human MxA protein belongs to the dynamin superfamily of large GTPases. It exhibits antiviral activity against a variety of RNA viruses, including Thogoto virus, an influenza virus-like orthomyxovirus transmitted by ticks. Here, we report that MxA blocks the transport of Thogoto virus nucleocapsids into the nucleus, thereby preventing transcription of the viral genome. This interaction can be abolished by a mAb that neutralizes the antiviral activity of MxA. Our results reveal an antiviral mechanism whereby an interferon-induced protein traps the incoming virus and interferes with proper transport of the viral genome to its ultimate target compartment within the infected cell.

MeSH Terms
3T3 Cells Animals Antibodies, Monoclonal/pharmacology Antiviral Agents/pharmacology Cell Line Cell Nucleus/physiology,virology Cricetinae Cycloheximide/pharmacology Enzyme Induction GTP Phosphohydrolases/metabolism GTP-Binding Proteins Genome, Viral Humans Interferons/pharmacology,physiology Mice Models, Biological Myxovirus Resistance Proteins Nucleocapsid Proteins/genetics,metabolism Protein Biosynthesis Proteins/metabolism Thogotovirus/genetics,physiology Transcription, Genetic Virus Replication
Chemicals
Antibodies, Monoclonal Antiviral Agents MX1 protein, human Myxovirus Resistance Proteins Nucleocapsid Proteins Proteins Interferons Cycloheximide GTP Phosphohydrolases GTP-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kochs G
Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, D-79008 Freiburg, Germany.
Haller O
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.5.2082
Published
1999-03-02
Pages
2082-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC26740
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.96.5.2082 PMC Full Text View journal details
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