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PMID: 10024543 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role of Bordetella pertussis virulence factors in adherence to epithelial cell lines derived from the human respiratory tract.

Infection and immunity ·Vol. 67 ·No. 3 ·1999-03-00 ·Pages 1056-62

van den Berg BM, Beekhuizen H, Willems RJ, Mooi FR, van Furth R

Abstract

During colonization of the respiratory tract by Bordetella pertussis, virulence factors contribute to adherence of the bacterium to the respiratory tract epithelium. In the present study, we examined the roles of the virulence factors filamentous hemagglutinin (FHA), fimbriae, pertactin (Prn), and pertussis toxin (PT) in the adherence of B. pertussis to cells of the human bronchial epithelial cell line NCI-H292 and of the laryngeal epithelial cell line HEp-2. Using B. pertussis mutant strains and purified FHA, fimbriae, Prn, and PT, we demonstrated that both fimbriae and FHA are involved in the adhesion of B. pertussis to laryngeal epithelial cells, whereas only FHA is involved in the adherence to bronchial epithelial cells. For PT and Prn, no role as adhesion factor was found. However, purified PT bound to both bronchial and laryngeal cells and as such reduced the adherence of B. pertussis to these cells. These data may imply that fimbriae play a role in infection of only the laryngeal mucosa, while FHA is the major factor in colonization of the entire respiratory tract.

MeSH Terms
Adhesins, Bacterial/physiology Bacterial Adhesion Bacterial Outer Membrane Proteins/physiology Bordetella pertussis/pathogenicity,physiology Bronchi/microbiology Cell Line Fimbriae, Bacterial/physiology Hemagglutinins/physiology Humans Pertussis Toxin Virulence Virulence Factors, Bordetella/toxicity
Chemicals
Adhesins, Bacterial Bacterial Outer Membrane Proteins Hemagglutinins Virulence Factors, Bordetella filamentous hemagglutinin adhesin, Bordetella pertussis pertactin Pertussis Toxin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
van den Berg B M
Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands. bvdberg@stad.dsl.nl
Beekhuizen H
Willems R J
Mooi F R
van Furth R
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.67.3.1056-1062.1999
Email
bvdberg@stad.dsl.nl
Published
1999-03-00
Pages
1056-62
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC96429
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.67.3.1056-1062.1999 PMC Full Text View journal details
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