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PMID: 7927760 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Virulence factors determine attachment and ingestion of nonopsonized and opsonized Bordetella pertussis by human monocytes.

Infection and immunity ·Vol. 62 ·No. 11 ·1994-11-00 ·Pages 4818-24

Hazenbos WL, van den Berg BM, van't Wout JW, Mooi FR, van Furth R

Abstract

In the present study, the role of virulence factors in and the effect of opsonization on the interactions between Bordetella pertussis and human monocytes were investigated. The methods used facilitated the distinction between attachment and ingestion of bacteria by monocytes. Nonopsonized virulent B. pertussis cells attached to monocytes. Nonopsonized B. pertussis mutant strains deficient in filamentous hemagglutinin, fimbriae, or pertactin exhibited a reduced adherence to monocytes compared with that of their respective parental strains. Nonopsonized avirulent B. pertussis cells did not attach to monocytes. These results led to the conclusion that fimbriae and pertactin are involved in the adherence of nonopsonized virulent B. pertussis cells to monocytes and confirm the role of filamentous hemagglutinin in this process. In the absence of opsonins, about 40% of the monocyte-associated virulent B. pertussis cells were ingested. When B. pertussis cells were preopsonized with inactivated normal serum, about 50% of the monocyte-associated virulent B. pertussis cells were phagocytosed and about 80% of the monocyte-associated avirulent B. pertussis cells were ingested. These results indicate that virulence factors inhibit opsonin-mediated ingestion of B. pertussis by monocytes.

MeSH Terms
Antibodies, Bacterial/immunology Bacterial Adhesion Bacterial Outer Membrane Proteins/metabolism Bordetella pertussis/immunology,pathogenicity Cell Adhesion Hemagglutinins/metabolism Humans Immunoglobulin G/immunology In Vitro Techniques Monocytes/cytology,immunology,microbiology Opsonin Proteins Phagocytosis Virulence Factors, Bordetella
Chemicals
Antibodies, Bacterial Bacterial Outer Membrane Proteins Hemagglutinins Immunoglobulin G Opsonin Proteins Virulence Factors, Bordetella pertactin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hazenbos W L
Department of Infectious Diseases, University Hospital, Leiden, The Netherlands.
van den Berg B M
van't Wout J W
Mooi F R
van Furth R
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.62.11.4818-4824.1994
Published
1994-11-00
Pages
4818-24
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC303192
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.62.11.4818-4824.1994 PMC Full Text View journal details
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