Molecular weight and subunit size of fatty acid synthetase from rabbit mammary gland.

The Biochemical journal ·Vol. 159 ·No. 1 ·1976-10-01 ·Pages 181-4

Paskin N, Mayer RJ

Abstract

Fatty acid synthetase purified from the mammary gland of the rabbit has a mol. wt. of 968000 as determined by gel filtration. The enzyme gave one band, corresponding to a mol.wt. of approx. 35000, on polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate and phenylmethanesulphonyl fluoride.

MeSH Terms

Animals Chromatography, Gel Electrophoresis, Polyacrylamide Gel Fatty Acid Synthases Female Mammary Glands, Animal/enzymology Molecular Weight Phenylmethylsulfonyl Fluoride Rabbits

Chemicals

Phenylmethylsulfonyl Fluoride Fatty Acid Synthases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Paskin N

Mayer R J

References (22)

22 references, click to expand

Presence of two polypeptide chains comprising fatty acid synthetase.
Proc Natl Acad Sci U S A. 1975 May;72(5):1940-4 PMID: 1098047
Subunit constitution of proteins: a table.
Arch Biochem Biophys. 1975 Feb;166(2):651-82 PMID: 1119814
Regulation of enzyme turnover during tissue differention. Studies on the effects of hormones on the turnover of fatty acid synthetase in rabbit mammary gland in organ culture.
Biochem J. 1975 May;148(2):309-20 PMID: 1156407
Evidence for an "active serine" in each fatty acid synthetase peptide.
Biochem Biophys Res Commun. 1976 Jan 26;68(2):379-86 PMID: 1252235
Fatty acid synthesis in adipose tissue. I. Purification and properties of a long chain fatty acid-synthesizing system.
J Biol Chem. 1961 Mar;236:663-8 PMID: 13767323
The condensation reaction of fatty acid biosynthesis. II. Requirement of the enzymes of the condensation reaction for fatty acid synthesis.
J Biol Chem. 1963 Apr;238:1255-61 PMID: 13948777
Biosynthesis of saturated fatty acids.
Fed Proc. 1961 Dec;20:941-51 PMID: 14467591
A FATTY ACID SYNTHETASE FROM E. COLI.
Proc Natl Acad Sci U S A. 1962 May;48(5):840-6 PMID: 16590950
Subunits of fatty acid synthetase complexes. Enzymatic activities and properties of the half-molecular weight nonidentical subunits of pigeon liver fatty acid synthetase.
J Biol Chem. 1975 Jun 25;250(12):4520-9 PMID: 237903
Purification and properties of 6-phosphogluconate dehydrogenase from rabbit mammary gland.
Biochem J. 1975 Nov;151(2):263-70 PMID: 3163
The purification and properties of the fatty acid synthetase of pigeon liver.
J Biol Chem. 1965 Oct;240(10):3736-46 PMID: 4378857
Acyl carrier protein.
Adv Enzymol Relat Areas Mol Biol. 1972;36:269-311 PMID: 4561013
Pantetheine-free mutants of the yeast fatty-acid-synthetase complex.
Eur J Biochem. 1973 Nov 15;39(2):353-62 PMID: 4590449
Fatty acid synthetase complex. Selective inactivation by phenylmethylsulphonyl fluoride.
Biochem Biophys Res Commun. 1973 Jul 2;53(1):334-41 PMID: 4741553
A simple method for preliminary immunodiffusion test of antigen-antibody systems having unknown ratios of reaction.
Anal Biochem. 1969 Feb;27(2):281-4 PMID: 4975046
Structure and function of the acyl carrier protein.
Fed Proc. 1966 Sep-Oct;25(5):1485-94 PMID: 5332188
Fatty acid synthetase from lactating rat mammary gland. I. Isolation and properties.
J Biol Chem. 1970 Jun;245(12):3209-17 PMID: 5464659
Fatty acid biosynthesis. V. Purification and characterisation of fatty acid synthetase from lactating-rabbit mammary gland.
Biochim Biophys Acta. 1970 Sep 8;210(3):371-87 PMID: 5528596
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
Localization of the central and peripheral SH-groups on the same polypeptide chain of yeast fatty acid synthetase.
Biochem Biophys Res Commun. 1976 Apr 19;69(4):893-9 PMID: 776182
One-step purification and properties of a two-peptide fatty acid synthetase from the uropygial gland of the goose.
Biochemistry. 1976 May 4;15(9):1948-57 PMID: 817736
Sodium dodecyl sulfate disc-gel electrophoresis pattern of protease-free chicken liver fatty acid synthetase.
Arch Biochem Biophys. 1976 May;174(1):136-42 PMID: 938047

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1590181

Published

1976-10-01

Pages

181-4

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1164055

Subset

External Links

PubMed source DOI: 10.1042/bj1590181 PMC Full Text View journal details

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Molecular weight and subunit size of fatty acid synthetase from rabbit mammary gland.

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