Home LiteratureArticle Details
PMID: 9989977 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of dendritic cells with skin endothelium: A new perspective on immunosurveillance.

The Journal of experimental medicine ·Vol. 189 ·No. 4 ·1999-02-15 ·Pages 627-36

Robert C, Fuhlbrigge RC, Kieffer JD, Ayehunie S, Hynes RO, Cheng G, Grabbe S, von Andrian UH, Kupper TS

Abstract

The goal of this study was to determine the mechanisms by which dendritic cells (DCs) in blood could interact with endothelium, a prerequisite to extravasation into tissues. Our results indicate that DCs express both HECA-452-reactive and nonreactive isoforms of P-selectin glycoprotein ligand 1 (PSGL-1) and can tether and roll efficiently on E- and P-selectin under flow conditions in vitro. Freshly isolated blood DCs were further observed to roll continuously along noninflamed murine dermal endothelium in vivo. This interaction is strictly dependent on endothelial selectins, as shown by experiments with blocking antibodies and with E- and P-selectin-deficient mice. We hypothesize that DCs in blood are constitutively poised at the interface of blood and skin, ready to extravasate upon induction of inflammation, and we showed that cutaneous inflammation results in a rapid recruitment of DCs from the blood to tissues. We propose that this is an important and previously unappreciated element of immunosurveillance.

MeSH Terms
Animals Blood Cells/immunology Bone Marrow Cells/physiology Cell Adhesion Cell Movement Cells, Cultured Dendritic Cells/immunology,physiology E-Selectin/genetics,physiology Ear, External Endothelium/immunology Hemorheology Humans Hypersensitivity, Delayed/immunology Immunologic Surveillance Immunomagnetic Separation Membrane Glycoproteins/analysis,physiology Mice Mice, Knockout Models, Immunological Oxazolone/toxicity P-Selectin/genetics,physiology Skin/immunology
Chemicals
E-Selectin Membrane Glycoproteins P-Selectin P-selectin ligand protein Oxazolone
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Robert C
Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115, USA.
Fuhlbrigge R C
Kieffer J D
Ayehunie S
Hynes R O
Cheng G
Grabbe S
von Andrian U H
Kupper T S
References (42)
42 references, click to expand
  1. GM-CSF and TNF-alpha cooperate in the generation of dendritic Langerhans cells.
    Nature. 1992 Nov 19;360(6401):258-61 PMID: 1279441
  2. Effect of in vivo interleukin-1 on adhesion molecule expression in normal human skin.
    J Invest Dermatol. 1992 Mar;98(3):384-7 PMID: 1372029
  3. Requirement for VLA-4 and VLA-5 integrins in lymphoma cells binding to and migration beneath stromal cells in culture.
    J Cell Biol. 1992 Nov;119(3):653-62 PMID: 1400595
  4. Differential expression of homing-associated adhesion molecules by T cell subsets in man.
    J Immunol. 1990 Nov 15;145(10):3247-55 PMID: 1700003
  5. Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins.
    Cell. 1991 May 31;65(5):859-73 PMID: 1710173
  6. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1.
    J Exp Med. 1991 Dec 1;174(6):1461-6 PMID: 1720810
  7. The dendritic cell system and its role in immunogenicity.
    Annu Rev Immunol. 1991;9:271-96 PMID: 1910679
  8. Endothelial, not hemodynamic, differences are responsible for preferential leukocyte rolling in rat mesenteric venules.
    Circ Res. 1991 Oct;69(4):1034-41 PMID: 1934331
  9. Rapid leukocyte accumulation by "spontaneous" rolling and adhesion in the exteriorized rabbit mesentery.
    Int J Microcirc Clin Exp. 1991 May;10(2):127-44 PMID: 2060996
  10. Long term effect on epidermal dendritic cells of four different types of exogenous inflammation.
    Acta Derm Venereol. 1987;67(4):305-9 PMID: 2445148
  11. Quantitative investigations of the adhesiveness of circulating polymorphonuclear leucocytes to blood vessel walls.
    J Physiol. 1972 Apr;222(2):447-74 PMID: 4624453
  12. Bone marrow-derived dendritic cells pulsed with synthetic tumour peptides elicit protective and therapeutic antitumour immunity.
    Nat Med. 1995 Dec;1(12):1297-302 PMID: 7489412
  13. Leukocyte rolling in venules of striated muscle and skin is mediated by P-selectin, not by L-selectin.
    Am J Physiol. 1994 Oct;267(4 Pt 2):H1637-42 PMID: 7524368
  14. Human blood contains two subsets of dendritic cells, one immunologically mature and the other immature.
    Immunology. 1994 Jul;82(3):487-93 PMID: 7525461
  15. The P-selectin glycoprotein ligand functions as a common human leukocyte ligand for P- and E-selectins.
    J Biol Chem. 1995 May 12;270(19):11662-70 PMID: 7538120
  16. Migration of dendritic cells in response to formyl peptides, C5a, and a distinct set of chemokines.
    J Immunol. 1995 Oct 1;155(7):3292-5 PMID: 7561021
  17. Control of lymphocyte recirculation in man. II. Differential regulation of the cutaneous lymphocyte-associated antigen, a tissue-selective homing receptor for skin-homing T cells.
    J Immunol. 1993 Feb 1;150(3):1122-36 PMID: 7678617
  18. Traffic signals on endothelium for lymphocyte recirculation and leukocyte emigration.
    Annu Rev Physiol. 1995;57:827-72 PMID: 7778885
  19. Rapid dendritic cell recruitment is a hallmark of the acute inflammatory response at mucosal surfaces.
    J Exp Med. 1994 Apr 1;179(4):1331-6 PMID: 8145044
  20. Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins.
    Cell. 1996 Feb 23;84(4):563-74 PMID: 8598043
  21. Lymphocyte homing and homeostasis.
    Science. 1996 Apr 5;272(5258):60-6 PMID: 8600538
  22. The alpha(1,3)fucosyltransferase Fuc-TVII controls leukocyte trafficking through an essential role in L-, E-, and P-selectin ligand biosynthesis.
    Cell. 1996 Aug 23;86(4):643-53 PMID: 8752218
  23. Molecular and functional characteristics of dendritic cells generated from highly purified CD14+ peripheral blood monocytes.
    J Immunol. 1996 Nov 1;157(9):3850-9 PMID: 8892615
  24. Sialylated, fucosylated ligands for L-selectin expressed on leukocytes mediate tethering and rolling adhesions in physiologic flow conditions.
    J Cell Biol. 1996 Nov;135(3):837-48 PMID: 8909555
  25. Dendritic cells are recruited into the airway epithelium during the inflammatory response to a broad spectrum of stimuli.
    J Exp Med. 1996 Dec 1;184(6):2429-32 PMID: 8976199
  26. P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues.
    Nature. 1997 Jan 2;385(6611):81-3 PMID: 8985251
  27. A skin homing molecule defines the langerhans cell progenitor in human peripheral blood.
    J Exp Med. 1997 Mar 17;185(6):1131-6 PMID: 9091586
  28. Human mucosal addressin cell adhesion molecule-1 is preferentially expressed in intestinal tract and associated lymphoid tissue.
    Am J Pathol. 1997 Jul;151(1):97-110 PMID: 9212736
  29. Human immunodeficiency virus-1 entry into purified blood dendritic cells through CC and CXC chemokine coreceptors.
    Blood. 1997 Aug 15;90(4):1379-86 PMID: 9269754
  30. The migratory behavior of murine CD4+ cells of memory phenotype.
    Eur J Immunol. 1997 Sep;27(9):2225-32 PMID: 9341763
  31. Dendritic cells: unique leukocyte populations which control the primary immune response.
    Blood. 1997 Nov 1;90(9):3245-87 PMID: 9345009
  32. Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells.
    Nature. 1997 Oct 30;389(6654):978-81 PMID: 9353122
  33. Molecular mechanisms of lymphocyte homing to peripheral lymph nodes.
    J Exp Med. 1998 Jan 19;187(2):205-16 PMID: 9432978
  34. Chemokines--chemotactic cytokines that mediate inflammation.
    N Engl J Med. 1998 Feb 12;338(7):436-45 PMID: 9459648
  35. Complexity and differential expression of carbohydrate epitopes associated with L-selectin recognition of high endothelial venules.
    Am J Pathol. 1998 Feb;152(2):469-77 PMID: 9466573
  36. Flexible programs of chemokine receptor expression on human polarized T helper 1 and 2 lymphocytes.
    J Exp Med. 1998 Mar 16;187(6):875-83 PMID: 9500790
  37. Dendritic cells and the control of immunity.
    Nature. 1998 Mar 19;392(6673):245-52 PMID: 9521319
  38. Expression of maturation-/migration-related molecules on human dendritic cells from blood and skin.
    Immunobiology. 1998 Mar;198(5):568-87 PMID: 9561374
  39. Selective recruitment of immature and mature dendritic cells by distinct chemokines expressed in different anatomic sites.
    J Exp Med. 1998 Jul 20;188(2):373-86 PMID: 9670049
  40. Differential regulation of chemokine receptors during dendritic cell maturation: a model for their trafficking properties.
    J Immunol. 1998 Aug 1;161(3):1083-6 PMID: 9686565
  41. Hematopoietic progenitor cell rolling in bone marrow microvessels: parallel contributions by endothelial selectins and vascular cell adhesion molecule 1.
    J Exp Med. 1998 Aug 3;188(3):465-74 PMID: 9687524
  42. Rapid and coordinated switch in chemokine receptor expression during dendritic cell maturation.
    Eur J Immunol. 1998 Sep;28(9):2760-9 PMID: 9754563
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.189.4.627
Published
1999-02-15
Pages
627-36
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192925
Subset
IM
Corrections
CommentIn
PMID: 9989975
External Links
PubMed source DOI: 10.1084/jem.189.4.627 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2