Evaluation of the ultrasensitive Roche Amplicor HIV-1 monitor assay for quantitation of human immunodeficiency virus type 1 RNA.

Journal of clinical microbiology ·Vol. 37 ·No. 3 ·1999-03-00 ·Pages 792-5

Erali M, Hillyard DR

Abstract

The ultrasensitive Amplicor HIV-1 Monitor test (Roche Diagnostic Systems) was evaluated for precision, linearity, and sensitivity and was compared to the standard Amplicor assay. The ultrasensitive assay reliably quantified samples in the range from 50 to 50,000 human immunodeficiency virus type 1 RNA copies/ml with acceptable correlation with the standard Amplicor test.

MeSH Terms

Acquired Immunodeficiency Syndrome/blood HIV-1/isolation & purification Humans RNA, Viral/blood Regression Analysis Reproducibility of Results Sensitivity and Specificity Viral Load Virology/methods

Chemicals

RNA, Viral

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Erali M

ARUP Institute for Clinical and Experimental Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah 84108, USA.

Hillyard D R

References (4)

4 references, click to expand

HIV viral load markers in clinical practice.
Nat Med. 1996 Jun;2(6):625-9 PMID: 8640545
Changes in plasma HIV RNA levels and CD4+ lymphocyte counts predict both response to antiretroviral therapy and therapeutic failure. VA Cooperative Study Group on AIDS.
Ann Intern Med. 1997 Jun 15;126(12):939-45 PMID: 9182470
Plasma viral load and CD4+ lymphocytes as prognostic markers of HIV-1 infection.
Ann Intern Med. 1997 Jun 15;126(12):946-54 PMID: 9182471
Clinical comparison of an enhanced-sensitivity branched-DNA assay and reverse transcription-PCR for quantitation of human immunodeficiency virus type 1 RNA in plasma.
J Clin Microbiol. 1998 Mar;36(3):716-20 PMID: 9508301

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/JCM.37.3.792-795.1999

Published

1999-03-00

Pages

792-5

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC84558

Subset

External Links

PubMed source DOI: 10.1128/JCM.37.3.792-795.1999 PMC Full Text View journal details

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Evaluation of the ultrasensitive Roche Amplicor HIV-1 monitor assay for quantitation of human immunodeficiency virus type 1 RNA.

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