Incomplete inhibition of the immunoglobulin G-binding behavior of nitrated protein A.

Infection and immunity ·Vol. 21 ·No. 2 ·1978-08-00 ·Pages 659-62

Sloan GJ, Butler JE

Abstract

Nitration of protein A with tetranitromethane incompletely blocks its Fc-binding ability for immunoglobulin G when measured by more sensitive methods of immunoprecipitation and when tested in the enzyme-linked immunosorbent assay. In addition, binding of protein A and blockage by nitration is heterogeneous and depends on the species origin of the immunoglobulin G.

MeSH Terms

Animals Binding Sites, Antibody Binding, Competitive Cattle Enzyme-Linked Immunosorbent Assay Goats Guinea Pigs Immunodiffusion Immunoglobulin G Rabbits Staphylococcal Protein A/immunology Swine

Chemicals

Immunoglobulin G Staphylococcal Protein A

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sloan G J

Butler J E

References (8)

8 references, click to expand

Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes.
Biochim Biophys Acta. 1971 Dec 28;251(3):427-34 PMID: 11452886
Amplification of the enzyme-linked immunosorbent assay (ELISA) in the detection of class-specific antibodies.
J Immunol Methods. 1978;20:365-83 PMID: 306397
Protein A from Staphylococcus aureus. XIV. The effect of nitration of protein A with tetranitromethane and subsequent reduction.
J Immunol. 1973 Jun;110(6):1562-9 PMID: 4197304
Preparation of bovine immunoglobulins and free secretory component and their specific antisera.
J Dairy Sci. 1972 Feb;55(2):151-64 PMID: 4627643
Detection of type-specific pneumococcal antigens by counterimmunoelectrophoresis. I. Methodology and immunologic properties of pneumococcal antigens.
J Lab Clin Med. 1973 May;81(5):770-7 PMID: 4633404
Polymer-induced precipitation of antigen-antibody complexes: "precipiplex" reactions.
Immunochemistry. 1971 May;8(5):413-21 PMID: 4996448
Serum antigens of cattle. II. Immunogenetics of two immunoglobulin allotypes.
J Immunol. 1971 Jul;107(1):227-35 PMID: 4997113
Evaluation of enzyme-linked immunosorbent assay for quantitation by subclass for bovine antibodies.
Am J Vet Res. 1978 Jun;39(6):935-41 PMID: 96709

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.21.2.659-662.1978

Published

1978-08-00

Pages

659-62

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC422043

Subset

External Links

PubMed source DOI: 10.1128/iai.21.2.659-662.1978 PMC Full Text View journal details

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Incomplete inhibition of the immunoglobulin G-binding behavior of nitrated protein A.

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