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PMID: 9927696 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Variation in expression of the Haemophilus influenzae HMW adhesins: a prokaryotic system reminiscent of eukaryotes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 3 ·1999-02-02 ·Pages 1077-82

Dawid S, Barenkamp SJ, St Geme JW

Abstract

Expression of a number of eukaryotic genes is regulated by long stretches of tandem repeats located within the 5' untranslated region of the particular gene. In this study, we describe a regulatory system in Haemophilus influenzae with striking similarities to those found in eukaryotes. We show that expression of the HMW1 and HMW2 adhesins varies based on the number of 7-bp tandem repeats in the hmw1A and hmw2A promoters. The repeats lie between two separate transcription initiation sites and exert a repressive effect, such that increases in repeat number result in step-wise decreases in levels of specific mRNA and protein production and vice versa. The range of expression of HMW1 and HMW2 varies between very weak and very strong, with a series of gradations in between. Variation in the number of repeats in the hmw1A and hmw2A promoters occurs in individual colonies passaged in vitro, in an animal model of infection, and during natural infection in humans. This system of regulation is unique in prokaryotes and likely enhances the pathogenicity of the organism by increasing adaptive potential.

MeSH Terms
Adhesins, Bacterial/genetics Animals Bacterial Proteins/genetics Base Sequence Chinchilla/microbiology Genetic Variation Haemophilus influenzae/genetics,isolation & purification Humans Molecular Sequence Data Polymerase Chain Reaction Promoter Regions, Genetic RNA, Bacterial/genetics,isolation & purification Repetitive Sequences, Nucleic Acid Sequence Deletion Transcription, Genetic Untranslated Regions
Chemicals
Adhesins, Bacterial Bacterial Proteins HMW1 protein, bacteria HMW2 protein, bacteria RNA, Bacterial Untranslated Regions
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dawid S
Edward Mallinckrodt Department of Pediatrics and Molecular Microbiology, Washington University School of Medicine, Division of Infectious Diseases, St. Louis Children's Hospital, St. Louis, MO 63110, USA.
Barenkamp S J
St Geme J W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.3.1077
Published
1999-02-02
Pages
1077-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC15353
Subset
IM
Grants
NIDCD NIH HHS · R01 DC002873 · United States
NIDCD NIH HHS · ARO1 DC-02873 · United States
External Links
PubMed source DOI: 10.1073/pnas.96.3.1077 PMC Full Text View journal details
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