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PMID: 9889245 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Gram type-specific broad-range PCR amplification for rapid detection of 62 pathogenic bacteria.

Journal of clinical microbiology ·Vol. 37 ·No. 2 ·1999-02-00 ·Pages 464-6

Klausegger A, Hell M, Berger A, Zinober K, Baier S, Jones N, Sperl W, Kofler B

Abstract

Broad-range PCR has proven to be useful for the detection of bacteria. A set of broad-range PCR primers directed against conserved regions in the 16S rRNA gene was designed to specifically amplify either gram-positive or gram-negative bacteria. The gram type-specific broad-range PCR correctly classified all 62 pathogenic species tested.

MeSH Terms

DNA Primers DNA, Bacterial/analysis DNA, Ribosomal/analysis Genes, rRNA Gram-Negative Bacteria/classification,genetics,isolation & purification Gram-Negative Bacterial Infections/microbiology Gram-Positive Bacteria/classification,genetics,isolation & purification Gram-Positive Bacterial Infections/microbiology Humans Polymerase Chain Reaction/methods RNA, Ribosomal, 16S/genetics Species Specificity

Chemicals

DNA Primers DNA, Bacterial DNA, Ribosomal RNA, Ribosomal, 16S

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Klausegger A

Children's Hospital, General Hospital Salzburg, Salzburg, Austria.

Hell M

Berger A

Zinober K

Baier S

Jones N

Sperl W

Kofler B

References (8)

8 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/JCM.37.2.464-466.1999

Published

1999-02-00

Pages

464-6

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC84344

Subset

Corrections

ErratumIn

External Links

PubMed source DOI: 10.1128/JCM.37.2.464-466.1999 PMC Full Text View journal details

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