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PMID: 9882390 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Polyomavirus large T antigen binds the transcriptional coactivator protein p300.

Journal of virology ·Vol. 73 ·No. 2 ·1999-02-00 ·Pages 1734-9

Nemethova M, Wintersberger E

Abstract

Using coimmunoprecipitation and glutathione S-transferase pulldown experiments, we found that polyomavirus large T antigen binds to p300 in vivo and in vitro. The N-terminal region of the viral protein, including the pRB binding motif, was dispensable for this interaction, which involved several regions within the C-terminal half of the large T antigen. Interestingly, anti-T antibody coimmunoprecipitated a subspecies of p300 which has high histone acetyltransferase activity.

MeSH Terms
3T3 Cells Acetyltransferases/metabolism Animals Antigens, Polyomavirus Transforming/metabolism Cell Cycle Proteins/metabolism Cell Line, Transformed E1A-Associated p300 Protein Histone Acetyltransferases Mice Nuclear Proteins/metabolism Trans-Activators/metabolism Transcription Factors p300-CBP Transcription Factors
Chemicals
Antigens, Polyomavirus Transforming Cell Cycle Proteins Nuclear Proteins Trans-Activators Transcription Factors Acetyltransferases E1A-Associated p300 Protein Ep300 protein, mouse Histone Acetyltransferases p300-CBP Transcription Factors p300-CBP-associated factor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nemethova M
Institut für Molekularbiologie der Universität Wien, Wiener Biozentrum, A-1030 Vienna, Austria.
Wintersberger E
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.73.2.1734-1739.1999
Published
1999-02-00
Pages
1734-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC104009
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.73.2.1734-1739.1999 PMC Full Text View journal details
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