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PMID: 9882370 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of epstein-barr virus nuclear antigen 1 is associated with enhanced expression of CD25 in the Hodgkin cell line L428.

Journal of virology ·Vol. 73 ·No. 2 ·1999-02-00 ·Pages 1630-6

Kube D, Vockerodt M, Weber O, Hell K, Wolf J, Haier B, Grässer FA, Müller-Lantzsch N, Kieff E, Diehl V, Tesch H

Abstract

Epstein-Barr virus is associated with several human malignancies including Burkitt's lymphoma, nasopharyngeal carcinoma, and Hodgkin's disease (HD). To examine the effect of Epstein-Barr virus nuclear antigen 1 (EBNA-1) in the pathogenesis of HD, we transfected the gene into the HD cell line L428. EBNA-1 expression was associated with significantly enhanced CD25 expression (interleukin 2 [IL-2]-receptor alpha chain) in transient and stably transfected L428 cells but did not affect the expression of IL-2 receptor beta and gamma chains. There was no up-regulation of the B-cell activation molecules CD23, CD30, CD39, CD40, CD44, CD71, and CD54 (intercellular adhesion molecule 1) or enhanced production of IL-6, IL-10, lymphotoxin alpha, and the soluble form of CD25. Stable EBNA-1-expressing L428 cells were nontumorigenic in SCID mice but showed enhanced lymphoma development in nonobese diabetic-SCID mice compared to mock-transfected cells.

MeSH Terms
Animals Antigens, CD/analysis Carcinogenicity Tests Cytokines/metabolism Epstein-Barr Virus Nuclear Antigens/biosynthesis,genetics Gene Expression Herpesvirus 4, Human/genetics,metabolism Hodgkin Disease/immunology Humans Mice Mice, SCID Receptors, Interleukin-2/biosynthesis Transfection Tumor Cells, Cultured
Chemicals
Antigens, CD Cytokines Epstein-Barr Virus Nuclear Antigens Receptors, Interleukin-2
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Kube D
Klinik für Innere Medizin I, Cologne, Germany. ail14@pop.rrz.uni-koln.de
Vockerodt M
Weber O
Hell K
Wolf J
Haier B
Grässer F A
Müller-Lantzsch N
Kieff E
Diehl V
Tesch H
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.73.2.1630-1636.1999
Email
ail14@pop.rrz.uni-koln.de
Published
1999-02-00
Pages
1630-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC103989
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.73.2.1630-1636.1999 PMC Full Text View journal details
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