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PMID: 9880572 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

ATP released from astrocytes mediates glial calcium waves.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 19 ·No. 2 ·1999-01-15 ·Pages 520-8

Guthrie PB, Knappenberger J, Segal M, Bennett MV, Charles AC, Kater SB

Abstract

Calcium waves represent a widespread form of intercellular communication. Although they have been thought for a long time to require gap junctions, we recently demonstrated that mouse cortical astrocytes use an extracellular messenger for calcium wave propagation. The present experiments identify ATP as a major extracellular messenger in this system. Medium collected from astrocyte cultures during (but not before) calcium wave stimulation contains ATP. The excitatory effects of medium samples and of ATP are blocked by purinergic receptor antagonists and by pretreatment with apyrase; these same purinergic receptor antagonists block propagation of electrically evoked calcium waves. ATP, applied at the concentration measured in medium samples, evokes responses that are qualitatively and quantitatively similar to those evoked by those medium samples. These data implicate ATP as an important transmitter between CNS astrocytes.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Animals, Newborn Apyrase/metabolism Astrocytes/metabolism Calcium Signaling/physiology Cells, Cultured Culture Media Electric Stimulation Extracellular Space/drug effects,physiology Image Processing, Computer-Assisted Luminescent Measurements Mice Purinergic Antagonists
Chemicals
Culture Media Purinergic Antagonists Adenosine Triphosphate Apyrase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Guthrie P B
Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, Utah 84132, USA.
Knappenberger J
Segal M
Bennett M V
Charles A C
Kater S B
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1999-01-15
Pages
520-8
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6782195
Subset
IM
Grants
NINDS NIH HHS · NS37024 · United States
External Links
PubMed source PMC Full Text View journal details
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