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PMID: 9870947 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

EAT-4, a homolog of a mammalian sodium-dependent inorganic phosphate cotransporter, is necessary for glutamatergic neurotransmission in caenorhabditis elegans.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 19 ·No. 1 ·1999-01-01 ·Pages 159-67

Lee RY, Sawin ER, Chalfie M, Horvitz HR, Avery L

Abstract

The Caenorhabditis elegans gene eat-4 affects multiple glutamatergic neurotransmission pathways. We find that eat-4 encodes a protein similar in sequence to a mammalian brain-specific sodium-dependent inorganic phosphate cotransporter I (BNPI). Like BNPI in the rat CNS, eat-4 is expressed predominantly in a specific subset of neurons, including several proposed to be glutamatergic. Loss-of-function mutations in eat-4 cause defective glutamatergic chemical transmission but appear to have little effect on other functions of neurons. Our data suggest that phosphate ions imported into glutamatergic neurons through transporters such as EAT-4 and BNPI are required specifically for glutamatergic neurotransmission.

MeSH Terms
Amino Acid Sequence Animals Animals, Genetically Modified Brain/metabolism Caenorhabditis elegans/genetics,metabolism Carrier Proteins/metabolism Cloning, Molecular Feeding Behavior/physiology Genes, Helminth Genetic Code Glutamic Acid/metabolism Mechanoreceptors/physiology Membrane Proteins/metabolism Molecular Sequence Data Rats Sodium-Phosphate Cotransporter Proteins Symporters Synaptic Transmission/physiology
Chemicals
Carrier Proteins Membrane Proteins Sodium-Phosphate Cotransporter Proteins Symporters Glutamic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lee R Y
Department of Molecular Biology and Oncology, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9148, USA.
Sawin E R
Chalfie M
Horvitz H R
Avery L
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1999-01-01
Pages
159-67
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC3759158
Subset
IM
Grants
NIGMS NIH HHS · R01 GM024663 · United States
NHLBI NIH HHS · HL46154 · United States
NHLBI NIH HHS · R01 HL046154 · United States
NIGMS NIH HHS · R37 GM024663 · United States
NHLBI NIH HHS · R37 HL046154 · United States
NIGMS NIH HHS · GM24663 · United States
External Links
PubMed source PMC Full Text View journal details
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