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PMID: 9869634 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Reprogramming of early embryonic blastomeres into endodermal progenitors by a Caenorhabditis elegans GATA factor.

Genes & development ·Vol. 12 ·No. 24 ·1998-12-15 ·Pages 3809-14

Zhu J, Fukushige T, McGhee JD, Rothman JH

Abstract

The END-1 GATA factor has been implicated in specifying endoderm in Caenorhabditis elegans and is the earliest known zygotic protein expressed in the lineage of E, the clonal endoderm progenitor. We report that ubiquitous end-1 expression during a critical period in embryogenesis causes all non-endodermal lineages to produce endoderm instead of ectoderm and/or mesoderm. END-1 expression bypasses the requirement for maternal SKN-1 and the maternal Wnt signaling pathway in endoderm formation. This suggests that a primary function of these maternal factors is to regulate zygotic end-1 expression, which is then sufficient to initiate the entire program for endoderm development.

MeSH Terms
Animals Animals, Genetically Modified Biomarkers/analysis Blastomeres/cytology Caenorhabditis elegans/cytology,embryology Caenorhabditis elegans Proteins Cell Differentiation Cell Lineage Cell Nucleus/metabolism Cytoplasmic Granules Cytoskeletal Proteins/genetics,physiology DNA-Binding Proteins Endoderm/cytology GATA Transcription Factors Helminth Proteins/genetics,physiology Intestines Proto-Oncogene Proteins/physiology Stem Cells/cytology Time Factors Trans-Activators Transcription Factors/genetics,physiology Wnt Proteins Zebrafish Proteins Zygote beta Catenin
Chemicals
Biomarkers Caenorhabditis elegans Proteins Cytoskeletal Proteins DNA-Binding Proteins END-1 protein, C elegans GATA Transcription Factors Helminth Proteins Proto-Oncogene Proteins Trans-Activators Transcription Factors Wnt Proteins Zebrafish Proteins beta Catenin skn-1 protein, C elegans
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zhu J
Department of Molecular, Cellular, and Developmental Biology and Neuroscience Research Institute, University of California, Santa Barbara, California 93106 USA.
Fukushige T
McGhee J D
Rothman J H
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.24.3809
Published
1998-12-15
Pages
3809-14
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC317268
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.12.24.3809 PMC Full Text View journal details
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