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PMID: 9863048 Published · ppublish English Journal Article

PCR-based random mutagenesis method using spiked oligonucleotides to randomize selected parts of a gene without any wild-type background.

BioTechniques ·Vol. 25 ·No. 6 ·1998-12-00 ·Pages 958, 962-5

Lanio T, Jeltsch A

Abstract

暂无摘要

MeSH Terms
Cloning, Molecular DNA-Directed DNA Polymerase Deoxyribonucleases, Type II Site-Specific Genetic Testing/methods Mutagenesis, Site-Directed Oligonucleotide Probes Plasmids Polymerase Chain Reaction/methods Restriction Mapping Transformation, Genetic
Chemicals
Oligonucleotide Probes Pfu DNA polymerase DNA-Directed DNA Polymerase Deoxyribonucleases, Type II Site-Specific GATATC-specific type II deoxyribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lanio T
Justus-Liebig Universität, Giessen, Germany.
Jeltsch A
Article Info
Journal
BioTechniques
Abbr.
Biotechniques
ISSN
0736-6205
DOI
10.2144/98256bm06
Published
1998-12-00
Pages
958, 962-5
Language
English
Region
England
NLM ID
8306785
Subset
IM
External Links
PubMed source DOI: 10.2144/98256bm06 View journal details
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