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PMID: 9862504 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning in Arabidopsis thaliana of a new protein phosphatase 2C (PP2C) with homology to ABI1 and ABI2.

Plant molecular biology ·Vol. 38 ·No. 5 ·1998-11-00 ·Pages 879-83

Rodriguez PL, Leube MP, Grill E

Abstract

We report the cloning of both the cDNA and the corresponding genomic sequence of a new PP2C from Arabidopsis thaliana, named AtP2C-HA (for homology to ABI1/ABI2). The AtP2C-HA cDNA contains an open reading frame of 1536 bp and encodes a putative protein of 511 amino acids with a predicted molecular mass of 55.7 kDa. The AtP2C-HA protein is composed of two domains, a C-terminal PP2C catalytic domain and a N-terminal extension of ca. 180 amino acid residues. The deduced amino acid sequence is 55% and 54% identical to ABI1 and ABI2, respectively. Comparison of the genomic structure of the ABI1, ABI2 and AtP2C-HA genes suggests that they belong to a multigene family. The expression of the AtP2C-HA gene is up-regulated by abscisic acid (ABA) treatment.

MeSH Terms
Abscisic Acid/pharmacology Amino Acid Sequence Arabidopsis/chemistry,enzymology,genetics Arabidopsis Proteins Base Sequence Blotting, Northern Blotting, Southern Cloning, Molecular DNA, Complementary/chemistry,genetics,isolation & purification DNA, Plant/chemistry,genetics Exons Gene Expression Regulation, Enzymologic/drug effects Gene Expression Regulation, Plant/drug effects Genes, Plant/genetics Introns Molecular Sequence Data Phosphoprotein Phosphatases/genetics Protein Phosphatase 2 Protein Phosphatase 2C RNA, Messenger/drug effects,genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Tissue Distribution
Chemicals
Arabidopsis Proteins DNA, Complementary DNA, Plant P2C-HA protein, Arabidopsis RNA, Messenger Saccharomyces cerevisiae Proteins Abscisic Acid ABI1 protein, Arabidopsis ABI2 protein, Arabidopsis PTC1 protein, S cerevisiae Phosphoprotein Phosphatases Protein Phosphatase 2 Protein Phosphatase 2C
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rodriguez P L
Instituto de Biologia Molecular y Celular de Plantas, Universidad Politécnica-CSIC, Valencia, Spain.
Leube M P
Grill E
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1006012218704
Published
1998-11-00
Pages
879-83
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
AJ003119
External Links
PubMed source DOI: 10.1023/a:1006012218704 View journal details
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